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A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas

During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation...

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Detalles Bibliográficos
Autores principales: Kurvari, Venkatesh, Grishin, Nick V., Snell, William J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175229/
https://www.ncbi.nlm.nih.gov/pubmed/9864368
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author Kurvari, Venkatesh
Grishin, Nick V.
Snell, William J.
author_facet Kurvari, Venkatesh
Grishin, Nick V.
Snell, William J.
author_sort Kurvari, Venkatesh
collection PubMed
description During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation for cell–cell fusion. We have identified a cDNA, gsp1, whose transcript levels are upregulated during flagellar adhesion. The GSP1 polypeptide is a novel, gamete-specific homeodomain protein, the first to be identified in an alga. Its homeodomain shows significant identity with several higher plant homeodomain proteins. Although encoded by a single copy gene present in cells of both mating types, immunoblot analysis showed that GSP1 was expressed in mating type (mt)+ gametes, but was not detectable in mt− gametes or in vegetative cells of either mating type. Moreover, GSP1 appeared late during gametogenesis, suggesting that it may function during adhesion with mt− gametes or after zygote formation. GSP1 is expressed in imp11, mt− mutant gametes, which have a lesion in the mid gene involved in sex determination and exhibit many phenotypic characteristics of mt+ gametes. Thus, gsp1 is negatively regulated by mid and is the first molecule to be identified in Chlamydomonas that shows sex-limited expression.
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spelling pubmed-21752292008-05-01 A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas Kurvari, Venkatesh Grishin, Nick V. Snell, William J. J Cell Biol Regular Articles During fertilization in Chlamydomonas, gametes of opposite mating types interact with each other through sex-specific adhesion molecules on their flagellar surfaces. Flagellar adhesion brings the cell bodies of the gametes into close contact and initiates a signal transduction pathway in preparation for cell–cell fusion. We have identified a cDNA, gsp1, whose transcript levels are upregulated during flagellar adhesion. The GSP1 polypeptide is a novel, gamete-specific homeodomain protein, the first to be identified in an alga. Its homeodomain shows significant identity with several higher plant homeodomain proteins. Although encoded by a single copy gene present in cells of both mating types, immunoblot analysis showed that GSP1 was expressed in mating type (mt)+ gametes, but was not detectable in mt− gametes or in vegetative cells of either mating type. Moreover, GSP1 appeared late during gametogenesis, suggesting that it may function during adhesion with mt− gametes or after zygote formation. GSP1 is expressed in imp11, mt− mutant gametes, which have a lesion in the mid gene involved in sex determination and exhibit many phenotypic characteristics of mt+ gametes. Thus, gsp1 is negatively regulated by mid and is the first molecule to be identified in Chlamydomonas that shows sex-limited expression. The Rockefeller University Press 1998-12-28 /pmc/articles/PMC2175229/ /pubmed/9864368 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Regular Articles
Kurvari, Venkatesh
Grishin, Nick V.
Snell, William J.
A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas
title A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas
title_full A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas
title_fullStr A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas
title_full_unstemmed A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas
title_short A Gamete-specific, Sex-limited Homeodomain Protein in Chlamydomonas
title_sort gamete-specific, sex-limited homeodomain protein in chlamydomonas
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175229/
https://www.ncbi.nlm.nih.gov/pubmed/9864368
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