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Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p

Two AAA family ATPases, NSF and p97, have been implicated in membrane fusion during assembly and inheritance of organelles of the secretory pathway. We have now investigated the roles of AAA ATPases in membrane fusion during assembly of the peroxisome, an organelle outside the classical secretory sy...

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Detalles Bibliográficos
Autores principales: Titorenko, Vladimir I., Rachubinski, Richard A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175277/
https://www.ncbi.nlm.nih.gov/pubmed/10953011
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author Titorenko, Vladimir I.
Rachubinski, Richard A.
author_facet Titorenko, Vladimir I.
Rachubinski, Richard A.
author_sort Titorenko, Vladimir I.
collection PubMed
description Two AAA family ATPases, NSF and p97, have been implicated in membrane fusion during assembly and inheritance of organelles of the secretory pathway. We have now investigated the roles of AAA ATPases in membrane fusion during assembly of the peroxisome, an organelle outside the classical secretory system. Here, we show that peroxisomal membrane fusion in the yeast Yarrowia lipolytica requires two AAA ATPases, Pex1p and Pex6p. Release of membrane- associated Pex1p and Pex6p drives the asymmetric priming of two fusion partners. The next step, peroxisome docking, requires release of Pex1p from one partner. Subsequent fusion of the peroxisomal membranes is independent of both Pex1p and Pex6p.
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spelling pubmed-21752772008-05-01 Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p Titorenko, Vladimir I. Rachubinski, Richard A. J Cell Biol Report Two AAA family ATPases, NSF and p97, have been implicated in membrane fusion during assembly and inheritance of organelles of the secretory pathway. We have now investigated the roles of AAA ATPases in membrane fusion during assembly of the peroxisome, an organelle outside the classical secretory system. Here, we show that peroxisomal membrane fusion in the yeast Yarrowia lipolytica requires two AAA ATPases, Pex1p and Pex6p. Release of membrane- associated Pex1p and Pex6p drives the asymmetric priming of two fusion partners. The next step, peroxisome docking, requires release of Pex1p from one partner. Subsequent fusion of the peroxisomal membranes is independent of both Pex1p and Pex6p. The Rockefeller University Press 2000-08-21 /pmc/articles/PMC2175277/ /pubmed/10953011 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Titorenko, Vladimir I.
Rachubinski, Richard A.
Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p
title Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p
title_full Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p
title_fullStr Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p
title_full_unstemmed Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p
title_short Peroxisomal Membrane Fusion Requires Two Aaa Family Atpases, Pex1p and Pex6p
title_sort peroxisomal membrane fusion requires two aaa family atpases, pex1p and pex6p
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175277/
https://www.ncbi.nlm.nih.gov/pubmed/10953011
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