Cargando…

Tenascin-C Suppresses Rho Activation

Cell binding to extracellular matrix (ECM) components changes cytoskeletal organization by the activation of Rho family GTPases. Tenascin-C, a developmentally regulated matrix protein, modulates cellular responses to other matrix proteins, such as fibronectin (FN). Here, we report that tenascin-C ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Wenk, Melissa B., Midwood, Kim S., Schwarzbauer, Jean E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175281/
https://www.ncbi.nlm.nih.gov/pubmed/10953015
_version_ 1782145451231805440
author Wenk, Melissa B.
Midwood, Kim S.
Schwarzbauer, Jean E.
author_facet Wenk, Melissa B.
Midwood, Kim S.
Schwarzbauer, Jean E.
author_sort Wenk, Melissa B.
collection PubMed
description Cell binding to extracellular matrix (ECM) components changes cytoskeletal organization by the activation of Rho family GTPases. Tenascin-C, a developmentally regulated matrix protein, modulates cellular responses to other matrix proteins, such as fibronectin (FN). Here, we report that tenascin-C markedly altered cell phenotype on a three-dimensional fibrin matrix containing FN, resulting in suppression of actin stress fibers and induction of actin-rich filopodia. This distinct morphology was associated with complete suppression of the activation of RhoA, a small GTPase that induces actin stress fiber formation. Enforced activation of RhoA circumvented the effects of tenascin. Effects of active Rho were reversed by a Rho inhibitor C3 transferase. Suppression of GTPase activation allows tenascin-C expression to act as a regulatory switch to reverse the effects of adhesive proteins on Rho function. This represents a novel paradigm for the regulation of cytoskeletal organization by ECM.
format Text
id pubmed-2175281
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21752812008-05-01 Tenascin-C Suppresses Rho Activation Wenk, Melissa B. Midwood, Kim S. Schwarzbauer, Jean E. J Cell Biol Report Cell binding to extracellular matrix (ECM) components changes cytoskeletal organization by the activation of Rho family GTPases. Tenascin-C, a developmentally regulated matrix protein, modulates cellular responses to other matrix proteins, such as fibronectin (FN). Here, we report that tenascin-C markedly altered cell phenotype on a three-dimensional fibrin matrix containing FN, resulting in suppression of actin stress fibers and induction of actin-rich filopodia. This distinct morphology was associated with complete suppression of the activation of RhoA, a small GTPase that induces actin stress fiber formation. Enforced activation of RhoA circumvented the effects of tenascin. Effects of active Rho were reversed by a Rho inhibitor C3 transferase. Suppression of GTPase activation allows tenascin-C expression to act as a regulatory switch to reverse the effects of adhesive proteins on Rho function. This represents a novel paradigm for the regulation of cytoskeletal organization by ECM. The Rockefeller University Press 2000-08-21 /pmc/articles/PMC2175281/ /pubmed/10953015 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Report
Wenk, Melissa B.
Midwood, Kim S.
Schwarzbauer, Jean E.
Tenascin-C Suppresses Rho Activation
title Tenascin-C Suppresses Rho Activation
title_full Tenascin-C Suppresses Rho Activation
title_fullStr Tenascin-C Suppresses Rho Activation
title_full_unstemmed Tenascin-C Suppresses Rho Activation
title_short Tenascin-C Suppresses Rho Activation
title_sort tenascin-c suppresses rho activation
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175281/
https://www.ncbi.nlm.nih.gov/pubmed/10953015
work_keys_str_mv AT wenkmelissab tenascincsuppressesrhoactivation
AT midwoodkims tenascincsuppressesrhoactivation
AT schwarzbauerjeane tenascincsuppressesrhoactivation