Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export

It is not known how Mex67p and Mtr2p, which form a heterodimer essential for mRNA export, transport mRNPs through the nuclear pore. Here, we show that the Mex67p/Mtr2p complex binds to all of the repeat types (GLFG, FXFG, and FG) found in nucleoporins. For this interaction, complex formation between...

Descripción completa

Detalles Bibliográficos
Autores principales: Sträßer, Katja, Baßler, Jochen, Hurt, Ed
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175290/
https://www.ncbi.nlm.nih.gov/pubmed/10952996
_version_ 1782145453362511872
author Sträßer, Katja
Baßler, Jochen
Hurt, Ed
author_facet Sträßer, Katja
Baßler, Jochen
Hurt, Ed
author_sort Sträßer, Katja
collection PubMed
description It is not known how Mex67p and Mtr2p, which form a heterodimer essential for mRNA export, transport mRNPs through the nuclear pore. Here, we show that the Mex67p/Mtr2p complex binds to all of the repeat types (GLFG, FXFG, and FG) found in nucleoporins. For this interaction, complex formation between Mex67p and Mtr2p has to occur. MEX67 and MTR2 also genetically interact with different types of repeat nucleoporins, such as Nup116p, Nup159p, Nsp1p, and Rip1p/Nup40p. These data suggest a model in which nuclear mRNA export requires the Mex67p/Mtr2p heterodimeric complex to directly contact several repeat nucleoporins, organized in different nuclear pore complex subcomplexes, as it carries the mRNP cargo through the nuclear pore.
format Text
id pubmed-2175290
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21752902008-05-01 Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export Sträßer, Katja Baßler, Jochen Hurt, Ed J Cell Biol Original Article It is not known how Mex67p and Mtr2p, which form a heterodimer essential for mRNA export, transport mRNPs through the nuclear pore. Here, we show that the Mex67p/Mtr2p complex binds to all of the repeat types (GLFG, FXFG, and FG) found in nucleoporins. For this interaction, complex formation between Mex67p and Mtr2p has to occur. MEX67 and MTR2 also genetically interact with different types of repeat nucleoporins, such as Nup116p, Nup159p, Nsp1p, and Rip1p/Nup40p. These data suggest a model in which nuclear mRNA export requires the Mex67p/Mtr2p heterodimeric complex to directly contact several repeat nucleoporins, organized in different nuclear pore complex subcomplexes, as it carries the mRNP cargo through the nuclear pore. The Rockefeller University Press 2000-08-21 /pmc/articles/PMC2175290/ /pubmed/10952996 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Sträßer, Katja
Baßler, Jochen
Hurt, Ed
Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export
title Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export
title_full Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export
title_fullStr Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export
title_full_unstemmed Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export
title_short Binding of the Mex67p/Mtr2p Heterodimer to Fxfg, Glfg, and Fg Repeat Nucleoporins Is Essential for Nuclear mRNA Export
title_sort binding of the mex67p/mtr2p heterodimer to fxfg, glfg, and fg repeat nucleoporins is essential for nuclear mrna export
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175290/
https://www.ncbi.nlm.nih.gov/pubmed/10952996
work_keys_str_mv AT straßerkatja bindingofthemex67pmtr2pheterodimertofxfgglfgandfgrepeatnucleoporinsisessentialfornuclearmrnaexport
AT baßlerjochen bindingofthemex67pmtr2pheterodimertofxfgglfgandfgrepeatnucleoporinsisessentialfornuclearmrnaexport
AT hurted bindingofthemex67pmtr2pheterodimertofxfgglfgandfgrepeatnucleoporinsisessentialfornuclearmrnaexport

Ejemplares similares