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A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus
Caulobacter crescentus is widely used as a powerful model system for the study of prokaryotic cell biology and development. Analysis of this organism is complicated by a limited selection of tools for genetic manipulation and inducible gene expression. This study reports the identification and funct...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175322/ https://www.ncbi.nlm.nih.gov/pubmed/17959646 http://dx.doi.org/10.1093/nar/gkm818 |
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author | Thanbichler, Martin Iniesta, Antonio A. Shapiro, Lucy |
author_facet | Thanbichler, Martin Iniesta, Antonio A. Shapiro, Lucy |
author_sort | Thanbichler, Martin |
collection | PubMed |
description | Caulobacter crescentus is widely used as a powerful model system for the study of prokaryotic cell biology and development. Analysis of this organism is complicated by a limited selection of tools for genetic manipulation and inducible gene expression. This study reports the identification and functional characterization of a vanillate-regulated promoter (P(van)) which meets all requirements for application as a multi-purpose expression system in Caulobacter, thus complementing the established xylose-inducible system (P(xyl)). Furthermore, we introduce a newly constructed set of integrating and replicating shuttle vectors that considerably facilitate cell biological and physiological studies in Caulobacter. Based on different narrow and broad-host range replicons, they offer a wide choice of promoters, resistance genes, and fusion partners for the construction of fluorescently or affinity-tagged proteins. Since many of these constructs are also suitable for use in other bacteria, this work provides a comprehensive collection of tools that will enrich many areas of microbiological research. |
format | Text |
id | pubmed-2175322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21753222008-01-07 A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus Thanbichler, Martin Iniesta, Antonio A. Shapiro, Lucy Nucleic Acids Res Methods Online Caulobacter crescentus is widely used as a powerful model system for the study of prokaryotic cell biology and development. Analysis of this organism is complicated by a limited selection of tools for genetic manipulation and inducible gene expression. This study reports the identification and functional characterization of a vanillate-regulated promoter (P(van)) which meets all requirements for application as a multi-purpose expression system in Caulobacter, thus complementing the established xylose-inducible system (P(xyl)). Furthermore, we introduce a newly constructed set of integrating and replicating shuttle vectors that considerably facilitate cell biological and physiological studies in Caulobacter. Based on different narrow and broad-host range replicons, they offer a wide choice of promoters, resistance genes, and fusion partners for the construction of fluorescently or affinity-tagged proteins. Since many of these constructs are also suitable for use in other bacteria, this work provides a comprehensive collection of tools that will enrich many areas of microbiological research. Oxford University Press 2007-11 2007-10-24 /pmc/articles/PMC2175322/ /pubmed/17959646 http://dx.doi.org/10.1093/nar/gkm818 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methods Online Thanbichler, Martin Iniesta, Antonio A. Shapiro, Lucy A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus |
title | A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus |
title_full | A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus |
title_fullStr | A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus |
title_full_unstemmed | A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus |
title_short | A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus |
title_sort | comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in caulobacter crescentus |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175322/ https://www.ncbi.nlm.nih.gov/pubmed/17959646 http://dx.doi.org/10.1093/nar/gkm818 |
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