USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES

The pipette method has proved a feasible method of obtaining one-cell pure cultures of anaerobes. Both bacilli and spores may be used as seeding material, but spores give a much higher percentage of positives. Boiling alone affords a sufficient degree of anaerobiosis to the medium for initiating one...

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Detalles Bibliográficos
Autor principal: Barber, M. A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1920
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180207/
https://www.ncbi.nlm.nih.gov/pubmed/19868446
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author Barber, M. A.
author_facet Barber, M. A.
author_sort Barber, M. A.
collection PubMed
description The pipette method has proved a feasible method of obtaining one-cell pure cultures of anaerobes. Both bacilli and spores may be used as seeding material, but spores give a much higher percentage of positives. Boiling alone affords a sufficient degree of anaerobiosis to the medium for initiating one-cell growths, and semisolid agar is the most convenient form of medium. Exposure to air during isolation apparently has no effect on the viability of spores of anaerobes, but young bacilli of some species suffer from a comparatively short exposure to free oxygen.
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spelling pubmed-21802072008-04-18 USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES Barber, M. A. J Exp Med Article The pipette method has proved a feasible method of obtaining one-cell pure cultures of anaerobes. Both bacilli and spores may be used as seeding material, but spores give a much higher percentage of positives. Boiling alone affords a sufficient degree of anaerobiosis to the medium for initiating one-cell growths, and semisolid agar is the most convenient form of medium. Exposure to air during isolation apparently has no effect on the viability of spores of anaerobes, but young bacilli of some species suffer from a comparatively short exposure to free oxygen. The Rockefeller University Press 1920-08-31 /pmc/articles/PMC2180207/ /pubmed/19868446 Text en Copyright © Copyright, 1920, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Barber, M. A.
USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES
title USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES
title_full USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES
title_fullStr USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES
title_full_unstemmed USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES
title_short USE OF THE SINGLE CELL METHOD IN OBTAINING PURE CULTURES OF ANAEROBES
title_sort use of the single cell method in obtaining pure cultures of anaerobes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180207/
https://www.ncbi.nlm.nih.gov/pubmed/19868446
work_keys_str_mv AT barberma useofthesinglecellmethodinobtainingpureculturesofanaerobes

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