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A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C

We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein found only in eukaryotes, exc...

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Autores principales: Homburg, S., Visochek, L., Moran, N., Dantzer, F., Priel, E., Asculai, E., Schwartz, D., Rotter, V., Dekel, N., Cohen-Armon, M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180227/
https://www.ncbi.nlm.nih.gov/pubmed/10908573
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author Homburg, S.
Visochek, L.
Moran, N.
Dantzer, F.
Priel, E.
Asculai, E.
Schwartz, D.
Rotter, V.
Dekel, N.
Cohen-Armon, M.
author_facet Homburg, S.
Visochek, L.
Moran, N.
Dantzer, F.
Priel, E.
Asculai, E.
Schwartz, D.
Rotter, V.
Dekel, N.
Cohen-Armon, M.
author_sort Homburg, S.
collection PubMed
description We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein found only in eukaryotes, exclusively catalyzes polyADP-ribosylation of DNA-binding proteins, thereby modulating their activity. Activation of PARP, reportedly induced by formation of DNA breaks, is involved in DNA transcription, replication, and repair. Our findings demonstrate an alternative mechanism: a fast activation of PARP, evoked by inositol 1,4,5,-trisphosphate–Ca(2+) mobilization, that does not involve DNA breaks. These findings identify PARP as a novel downstream target of phospholipase C, and unveil a novel fast signal–induced modification of DNA-binding proteins by polyADP-ribosylation.
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spelling pubmed-21802272008-05-01 A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C Homburg, S. Visochek, L. Moran, N. Dantzer, F. Priel, E. Asculai, E. Schwartz, D. Rotter, V. Dekel, N. Cohen-Armon, M. J Cell Biol Original Article We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein found only in eukaryotes, exclusively catalyzes polyADP-ribosylation of DNA-binding proteins, thereby modulating their activity. Activation of PARP, reportedly induced by formation of DNA breaks, is involved in DNA transcription, replication, and repair. Our findings demonstrate an alternative mechanism: a fast activation of PARP, evoked by inositol 1,4,5,-trisphosphate–Ca(2+) mobilization, that does not involve DNA breaks. These findings identify PARP as a novel downstream target of phospholipase C, and unveil a novel fast signal–induced modification of DNA-binding proteins by polyADP-ribosylation. The Rockefeller University Press 2000-07-24 /pmc/articles/PMC2180227/ /pubmed/10908573 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Homburg, S.
Visochek, L.
Moran, N.
Dantzer, F.
Priel, E.
Asculai, E.
Schwartz, D.
Rotter, V.
Dekel, N.
Cohen-Armon, M.
A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C
title A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C
title_full A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C
title_fullStr A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C
title_full_unstemmed A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C
title_short A Fast Signal–Induced Activation of Poly(Adp-Ribose) Polymerase: A Novel Downstream Target of Phospholipase C
title_sort fast signal–induced activation of poly(adp-ribose) polymerase: a novel downstream target of phospholipase c
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180227/
https://www.ncbi.nlm.nih.gov/pubmed/10908573
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