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A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS

1. Experiments have shown that tracheal exudate from two strains of laryngotracheitis of chickens from New Jersey and two from California when suspended in bouillon and passed through Berkefeld V filters will produce the disease. Two of six Berkefeld N filters allowed the etiological agent to pass,...

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Autor principal: Beach, J. R.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1931
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180297/
https://www.ncbi.nlm.nih.gov/pubmed/19869961
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author Beach, J. R.
author_facet Beach, J. R.
author_sort Beach, J. R.
collection PubMed
description 1. Experiments have shown that tracheal exudate from two strains of laryngotracheitis of chickens from New Jersey and two from California when suspended in bouillon and passed through Berkefeld V filters will produce the disease. Two of six Berkefeld N filters allowed the etiological agent to pass, whereas four did not. Attempts to produce the disease with Seitz filtrates were unsuccessful. These results demonstrate that laryngotracheitis is caused by a filtrable virus that because of its size or some other property does not pass readily through the finer filters. 2. It has been shown that the sera from fowls that have recovered from an infection with one of the New Jersey viruses will neutralize the same strain and also the one California strain tested. In order to demonstrate neutralization conclusively it was necessary to titrate samples of dried virus and in the tests to use approximately ten infecting doses. 3. The virus dried over calcium chloride for 10 days and then stored in the refrigerator for 60 days produced disease. Kept over calcium chloride for a month it was still active and when dried by Swift's method it remained alive for 5 months.
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spelling pubmed-21802972008-04-18 A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS Beach, J. R. J Exp Med Article 1. Experiments have shown that tracheal exudate from two strains of laryngotracheitis of chickens from New Jersey and two from California when suspended in bouillon and passed through Berkefeld V filters will produce the disease. Two of six Berkefeld N filters allowed the etiological agent to pass, whereas four did not. Attempts to produce the disease with Seitz filtrates were unsuccessful. These results demonstrate that laryngotracheitis is caused by a filtrable virus that because of its size or some other property does not pass readily through the finer filters. 2. It has been shown that the sera from fowls that have recovered from an infection with one of the New Jersey viruses will neutralize the same strain and also the one California strain tested. In order to demonstrate neutralization conclusively it was necessary to titrate samples of dried virus and in the tests to use approximately ten infecting doses. 3. The virus dried over calcium chloride for 10 days and then stored in the refrigerator for 60 days produced disease. Kept over calcium chloride for a month it was still active and when dried by Swift's method it remained alive for 5 months. The Rockefeller University Press 1931-11-30 /pmc/articles/PMC2180297/ /pubmed/19869961 Text en Copyright © Copyright, 1931, by The Rockefeller Institute for Medical Research New York This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Beach, J. R.
A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS
title A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS
title_full A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS
title_fullStr A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS
title_full_unstemmed A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS
title_short A FILTRABLE VIRUS, THE CAUSE OF INFECTIOUS LARYNGOTRACHEITIS OF CHICKENS
title_sort filtrable virus, the cause of infectious laryngotracheitis of chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180297/
https://www.ncbi.nlm.nih.gov/pubmed/19869961
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