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THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI

A chemical explanation has been provided for the production of opalescence in human and animal sera by extracts of certain Group A streptococci. Opalescence results from the selective liberation of specific lipids from α(1)-serum lipoprotein. The released lipids have been quantitatively separated th...

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Detalles Bibliográficos
Autor principal: Rowen, Robert
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180383/
https://www.ncbi.nlm.nih.gov/pubmed/14494659
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author Rowen, Robert
author_facet Rowen, Robert
author_sort Rowen, Robert
collection PubMed
description A chemical explanation has been provided for the production of opalescence in human and animal sera by extracts of certain Group A streptococci. Opalescence results from the selective liberation of specific lipids from α(1)-serum lipoprotein. The released lipids have been quantitatively separated through the relatively simple technique of ultracentrifugal flotation, and subsequently analyzed by chemical and chromatographic means. Esterified cholesterol constitutes by far the major low density reaction product, accounting for 85 to 90 per cent of the total lipid released. A small amount of phospholipid (apparently limited to lecithin) was the only other lipid consistently found in low density product fractions. Comparative kinetics of opalescence development and cholesterol release under varying conditions of pH, extract concentration, and temperature reveal that the two effects do not run exactly parallel but are, nevertheless, probably closely related manifestations of a specific enzymic degradation of serum lipoprotein. It has been suggested that enzymic action may occur at a site within the lipoprotein remote from the cholesterol esters themselves. Although the over-all reaction appears to be mediated by an enzyme present in many Group A streptococci, it is uncertain whether this enzyme acts directly on lipoprotein or acts by causing the activation of a serum enzyme which in turn attacks the lipoprotein.
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spelling pubmed-21803832008-04-17 THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI Rowen, Robert J Exp Med Article A chemical explanation has been provided for the production of opalescence in human and animal sera by extracts of certain Group A streptococci. Opalescence results from the selective liberation of specific lipids from α(1)-serum lipoprotein. The released lipids have been quantitatively separated through the relatively simple technique of ultracentrifugal flotation, and subsequently analyzed by chemical and chromatographic means. Esterified cholesterol constitutes by far the major low density reaction product, accounting for 85 to 90 per cent of the total lipid released. A small amount of phospholipid (apparently limited to lecithin) was the only other lipid consistently found in low density product fractions. Comparative kinetics of opalescence development and cholesterol release under varying conditions of pH, extract concentration, and temperature reveal that the two effects do not run exactly parallel but are, nevertheless, probably closely related manifestations of a specific enzymic degradation of serum lipoprotein. It has been suggested that enzymic action may occur at a site within the lipoprotein remote from the cholesterol esters themselves. Although the over-all reaction appears to be mediated by an enzyme present in many Group A streptococci, it is uncertain whether this enzyme acts directly on lipoprotein or acts by causing the activation of a serum enzyme which in turn attacks the lipoprotein. The Rockefeller University Press 1961-10-31 /pmc/articles/PMC2180383/ /pubmed/14494659 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rowen, Robert
THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI
title THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI
title_full THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI
title_fullStr THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI
title_full_unstemmed THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI
title_short THE RELEASE OF CHOLESTEROL ESTERS FROM SERUM LIPOPROTEINS BY EXTRACTS OF CERTAIN GROUP A STREPTOCOCCI
title_sort release of cholesterol esters from serum lipoproteins by extracts of certain group a streptococci
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180383/
https://www.ncbi.nlm.nih.gov/pubmed/14494659
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