THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL

Freezing of the chorioallantoic membrane after brief fixation in formalin preserves antigenicity of cellular components and allows penetration of ferritin-conjugated antibody. Dense aggregates of viral antigen, presumed to be of the soluble type, were found in the nuclei of cells infected with influ...

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Detalles Bibliográficos
Autores principales: Morgan, Councilman, Hsu, Konrad C., Rifkind, Richard A., Knox, Alice W., Rose, Harry M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1961
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180384/
https://www.ncbi.nlm.nih.gov/pubmed/19867208
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author Morgan, Councilman
Hsu, Konrad C.
Rifkind, Richard A.
Knox, Alice W.
Rose, Harry M.
author_facet Morgan, Councilman
Hsu, Konrad C.
Rifkind, Richard A.
Knox, Alice W.
Rose, Harry M.
author_sort Morgan, Councilman
collection PubMed
description Freezing of the chorioallantoic membrane after brief fixation in formalin preserves antigenicity of cellular components and allows penetration of ferritin-conjugated antibody. Dense aggregates of viral antigen, presumed to be of the soluble type, were found in the nuclei of cells infected with influenza virus. Intracytoplasmic antigen, on the other hand, was widely dispersed. The experimental observations are consistent with the hypothesis that soluble antigen diffuses into the cytoplasm through intact nuclear membranes. Nucleoli do not appear to be the sites of antigen synthesis.
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spelling pubmed-21803842008-04-17 THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL Morgan, Councilman Hsu, Konrad C. Rifkind, Richard A. Knox, Alice W. Rose, Harry M. J Exp Med Article Freezing of the chorioallantoic membrane after brief fixation in formalin preserves antigenicity of cellular components and allows penetration of ferritin-conjugated antibody. Dense aggregates of viral antigen, presumed to be of the soluble type, were found in the nuclei of cells infected with influenza virus. Intracytoplasmic antigen, on the other hand, was widely dispersed. The experimental observations are consistent with the hypothesis that soluble antigen diffuses into the cytoplasm through intact nuclear membranes. Nucleoli do not appear to be the sites of antigen synthesis. The Rockefeller University Press 1961-10-31 /pmc/articles/PMC2180384/ /pubmed/19867208 Text en Copyright © Copyright, 1961, by The Rockefeller Institute This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Morgan, Councilman
Hsu, Konrad C.
Rifkind, Richard A.
Knox, Alice W.
Rose, Harry M.
THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL
title THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL
title_full THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL
title_fullStr THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL
title_full_unstemmed THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL
title_short THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL
title_sort application of ferritin-conjugated antibody to electron microscopic studies of influenza virus in infected cells : ii. the interior of the cell
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180384/
https://www.ncbi.nlm.nih.gov/pubmed/19867208
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