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Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes
Rat antisera raised against anti-nuclease antibodies from mouse strains A/J and SJL detect strain-specific idiotypic determinants related to the antigen-combining site. These antisera have been used to investigate the genetic linkage and strain distribution of the anti- nuclease idiotypes. Despite t...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180713/ https://www.ncbi.nlm.nih.gov/pubmed/233902 |
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collection | PubMed |
description | Rat antisera raised against anti-nuclease antibodies from mouse strains A/J and SJL detect strain-specific idiotypic determinants related to the antigen-combining site. These antisera have been used to investigate the genetic linkage and strain distribution of the anti- nuclease idiotypes. Despite the existence of an H-2-linked immune response gene controlling the humoral response to nuclease, expression of the A/J anti-nuclease idiotype has been shown to be independent of genes in the H-2 region: the A/J idiotype was present in immune sera from strains A/J (H-2a) and A.BY (H-2b) but absent in sera from strains B10 (H-2b) and B10.A (H-2a). An analysis of the segregation of the A/J idiotype in offspring of the backcross (A/J x B10.A) x B10.A demonstrated linkage to the Ig-1e heavy chain allotype markers. In a small sample of backcross animals a very high apparent recombination frequency was observed, but further backcross analyses and progeny testing of putative recombinant animals will be required to substantiate this observation. Analysis of the A/J and SJL anti- nuclease idiotype markers in the BALB/c, CB.20, and BAB.14 strains indicate that these idiotypic markers may permit mapping of distinct variable region genes. |
format | Text |
id | pubmed-2180713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21807132008-04-17 Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes J Exp Med Articles Rat antisera raised against anti-nuclease antibodies from mouse strains A/J and SJL detect strain-specific idiotypic determinants related to the antigen-combining site. These antisera have been used to investigate the genetic linkage and strain distribution of the anti- nuclease idiotypes. Despite the existence of an H-2-linked immune response gene controlling the humoral response to nuclease, expression of the A/J anti-nuclease idiotype has been shown to be independent of genes in the H-2 region: the A/J idiotype was present in immune sera from strains A/J (H-2a) and A.BY (H-2b) but absent in sera from strains B10 (H-2b) and B10.A (H-2a). An analysis of the segregation of the A/J idiotype in offspring of the backcross (A/J x B10.A) x B10.A demonstrated linkage to the Ig-1e heavy chain allotype markers. In a small sample of backcross animals a very high apparent recombination frequency was observed, but further backcross analyses and progeny testing of putative recombinant animals will be required to substantiate this observation. Analysis of the A/J and SJL anti- nuclease idiotype markers in the BALB/c, CB.20, and BAB.14 strains indicate that these idiotypic markers may permit mapping of distinct variable region genes. The Rockefeller University Press 1977-03-01 /pmc/articles/PMC2180713/ /pubmed/233902 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes |
title | Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes |
title_full | Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes |
title_fullStr | Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes |
title_full_unstemmed | Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes |
title_short | Genetic control of the immune response to nuclease. V. Genetic linkage and strain distribution of anti-nuclease idiotypes |
title_sort | genetic control of the immune response to nuclease. v. genetic linkage and strain distribution of anti-nuclease idiotypes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180713/ https://www.ncbi.nlm.nih.gov/pubmed/233902 |