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Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincide...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180722/ https://www.ncbi.nlm.nih.gov/pubmed/233914 |
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collection | PubMed |
description | The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincident appearance in serial observations of pokeweed mitogen (PWM)-stimulated lymphocytes. The intensity of J-chain staining by fluorescent reagents in the stimulated cells synthesizing IgG was similar to that found in cells synthesizing IgA or IgM. Evidence was obtained that the presence of J chain in the IgG-producing cells did not reflect antecedent synthesis of IgA or IgM. T cells stimulated by phytohemagglutinin and PWM failed to show J-chain synthesis. Observations on lymphoid cell lines showed a similar parallelism between intracellular Ig and J-chain synthesis; no relation to surface Ig was found. |
format | Text |
id | pubmed-2180722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21807222008-04-17 Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines J Exp Med Articles The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincident appearance in serial observations of pokeweed mitogen (PWM)-stimulated lymphocytes. The intensity of J-chain staining by fluorescent reagents in the stimulated cells synthesizing IgG was similar to that found in cells synthesizing IgA or IgM. Evidence was obtained that the presence of J chain in the IgG-producing cells did not reflect antecedent synthesis of IgA or IgM. T cells stimulated by phytohemagglutinin and PWM failed to show J-chain synthesis. Observations on lymphoid cell lines showed a similar parallelism between intracellular Ig and J-chain synthesis; no relation to surface Ig was found. The Rockefeller University Press 1977-03-01 /pmc/articles/PMC2180722/ /pubmed/233914 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
title | Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
title_full | Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
title_fullStr | Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
title_full_unstemmed | Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
title_short | Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
title_sort | parallel synthesis of immunoglobulins and j chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180722/ https://www.ncbi.nlm.nih.gov/pubmed/233914 |