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Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines

The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincide...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180722/
https://www.ncbi.nlm.nih.gov/pubmed/233914
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collection PubMed
description The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincident appearance in serial observations of pokeweed mitogen (PWM)-stimulated lymphocytes. The intensity of J-chain staining by fluorescent reagents in the stimulated cells synthesizing IgG was similar to that found in cells synthesizing IgA or IgM. Evidence was obtained that the presence of J chain in the IgG-producing cells did not reflect antecedent synthesis of IgA or IgM. T cells stimulated by phytohemagglutinin and PWM failed to show J-chain synthesis. Observations on lymphoid cell lines showed a similar parallelism between intracellular Ig and J-chain synthesis; no relation to surface Ig was found.
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spelling pubmed-21807222008-04-17 Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines J Exp Med Articles The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincident appearance in serial observations of pokeweed mitogen (PWM)-stimulated lymphocytes. The intensity of J-chain staining by fluorescent reagents in the stimulated cells synthesizing IgG was similar to that found in cells synthesizing IgA or IgM. Evidence was obtained that the presence of J chain in the IgG-producing cells did not reflect antecedent synthesis of IgA or IgM. T cells stimulated by phytohemagglutinin and PWM failed to show J-chain synthesis. Observations on lymphoid cell lines showed a similar parallelism between intracellular Ig and J-chain synthesis; no relation to surface Ig was found. The Rockefeller University Press 1977-03-01 /pmc/articles/PMC2180722/ /pubmed/233914 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
title Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
title_full Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
title_fullStr Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
title_full_unstemmed Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
title_short Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
title_sort parallel synthesis of immunoglobulins and j chain in pokeweed mitogen- stimulated normal cells and in lymphoblastoid cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180722/
https://www.ncbi.nlm.nih.gov/pubmed/233914