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Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies

Monoclonal anti-dinitrophenyl antibodies generated in the splenic focus system from B cells of adult BALB/c mice were studied for the presence or absence of murine anti-T15 (M anti-T15) reactivity and for their ability to bind phosphorylcholine (PC). Two foci of the 680 clones analyzed bound PC, and...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180740/
https://www.ncbi.nlm.nih.gov/pubmed/301547
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description Monoclonal anti-dinitrophenyl antibodies generated in the splenic focus system from B cells of adult BALB/c mice were studied for the presence or absence of murine anti-T15 (M anti-T15) reactivity and for their ability to bind phosphorylcholine (PC). Two foci of the 680 clones analyzed bound PC, and one of these antibodies reacted with M anti-T15 and anti-Fab on a 1:1 weight basis. The discovery of a clonotype reactive with M anti-15 but not with rabbit anti-T15 (R anti-T15) serum, the converse of the R anti-T15+, M anti-T15- clonotype identified in the PC-specific repertoire, points to the novel idiotypic relationships which may be found among homogeneous antibodies binding diverse antigens. The R anti-T15-, M anti-T15+ clonotype may represent a distinct set of hypervariable region sequences inserted into the T15 framework or may be a somatic variant of the T15 germ-line sequence. In addition, the maximum frequency with which this clonotype occurs within the B-cell pool is estimated.
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spelling pubmed-21807402008-04-17 Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies J Exp Med Articles Monoclonal anti-dinitrophenyl antibodies generated in the splenic focus system from B cells of adult BALB/c mice were studied for the presence or absence of murine anti-T15 (M anti-T15) reactivity and for their ability to bind phosphorylcholine (PC). Two foci of the 680 clones analyzed bound PC, and one of these antibodies reacted with M anti-T15 and anti-Fab on a 1:1 weight basis. The discovery of a clonotype reactive with M anti-15 but not with rabbit anti-T15 (R anti-T15) serum, the converse of the R anti-T15+, M anti-T15- clonotype identified in the PC-specific repertoire, points to the novel idiotypic relationships which may be found among homogeneous antibodies binding diverse antigens. The R anti-T15-, M anti-T15+ clonotype may represent a distinct set of hypervariable region sequences inserted into the T15 framework or may be a somatic variant of the T15 germ-line sequence. In addition, the maximum frequency with which this clonotype occurs within the B-cell pool is estimated. The Rockefeller University Press 1977-07-01 /pmc/articles/PMC2180740/ /pubmed/301547 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
title Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
title_full Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
title_fullStr Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
title_full_unstemmed Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
title_short Novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
title_sort novel idiotypic and antigen-binding characteristics in two anti- dinitrophenyl monoclonal antibodies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2180740/
https://www.ncbi.nlm.nih.gov/pubmed/301547