Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines

The presence of phytohemagglutinin or pokeweed mitogen in cultures of human peripheral blood mononuclear cells in agar is known to stimulate the formation of lymphoid colonies. We now report that similar colonies can be induced in the absence of plant lectins upon addition of filtered and ultracentr...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2181895/
https://www.ncbi.nlm.nih.gov/pubmed/303689
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collection PubMed
description The presence of phytohemagglutinin or pokeweed mitogen in cultures of human peripheral blood mononuclear cells in agar is known to stimulate the formation of lymphoid colonies. We now report that similar colonies can be induced in the absence of plant lectins upon addition of filtered and ultracentrifuged conditioned medium (CM) obtained from certain human lymphoblastoid cell lines. Colony formation required at least 6 X 10(5) mononuclear cells per milliliter, and optimum results were obtained at concentrations of 1 X 10(6) cells/ml in the presence of 20% CM (50-500 colonies per 10(6) cells cultured). Individual cells within colonies displayed uniform morphological characteristics of lymphoid cells, and the majority formed rosettes with sheep erythrocytes, suggesting that they were of T-cell type.
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spelling pubmed-21818952008-04-17 Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines J Exp Med Articles The presence of phytohemagglutinin or pokeweed mitogen in cultures of human peripheral blood mononuclear cells in agar is known to stimulate the formation of lymphoid colonies. We now report that similar colonies can be induced in the absence of plant lectins upon addition of filtered and ultracentrifuged conditioned medium (CM) obtained from certain human lymphoblastoid cell lines. Colony formation required at least 6 X 10(5) mononuclear cells per milliliter, and optimum results were obtained at concentrations of 1 X 10(6) cells/ml in the presence of 20% CM (50-500 colonies per 10(6) cells cultured). Individual cells within colonies displayed uniform morphological characteristics of lymphoid cells, and the majority formed rosettes with sheep erythrocytes, suggesting that they were of T-cell type. The Rockefeller University Press 1977-12-01 /pmc/articles/PMC2181895/ /pubmed/303689 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
title Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
title_full Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
title_fullStr Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
title_full_unstemmed Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
title_short Lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
title_sort lymphocyte culture: induction of colonies by conditioned medium from human lymphoid cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2181895/
https://www.ncbi.nlm.nih.gov/pubmed/303689

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