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The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins
Membrane-bound antigens responsible for induction of a secondary allogeneic murine cytolytic T-cell (CTL) response have been obtained in a soluble, biologically active form by deoxycholate solubilization of tumor cell plasma membranes. The active proteins are soluble by the criteria of both ultracen...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184210/ https://www.ncbi.nlm.nih.gov/pubmed/305464 |
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collection | PubMed |
description | Membrane-bound antigens responsible for induction of a secondary allogeneic murine cytolytic T-cell (CTL) response have been obtained in a soluble, biologically active form by deoxycholate solubilization of tumor cell plasma membranes. The active proteins are soluble by the criteria of both ultracentrifugation and gel filtration. The immunological specificity of the induced CTL and removal of the activity from solution by treatment with B6 anti-P815 (anti-H-2d) antiserum and Protein A-Sepharose demonstrate that the CTL-inducing activity is dependent upon solubilized major histocompatibility complex antigens. |
format | Text |
id | pubmed-2184210 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21842102008-04-17 The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins J Exp Med Articles Membrane-bound antigens responsible for induction of a secondary allogeneic murine cytolytic T-cell (CTL) response have been obtained in a soluble, biologically active form by deoxycholate solubilization of tumor cell plasma membranes. The active proteins are soluble by the criteria of both ultracentrifugation and gel filtration. The immunological specificity of the induced CTL and removal of the activity from solution by treatment with B6 anti-P815 (anti-H-2d) antiserum and Protein A-Sepharose demonstrate that the CTL-inducing activity is dependent upon solubilized major histocompatibility complex antigens. The Rockefeller University Press 1978-03-01 /pmc/articles/PMC2184210/ /pubmed/305464 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins |
title | The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins |
title_full | The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins |
title_fullStr | The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins |
title_full_unstemmed | The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins |
title_short | The induction of secondary cytolytic T lymphocytes by solubilized membrane proteins |
title_sort | induction of secondary cytolytic t lymphocytes by solubilized membrane proteins |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184210/ https://www.ncbi.nlm.nih.gov/pubmed/305464 |