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Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera
The kinetics of bone marrow replacement was studied in W/WV mice implanted with gbj/bgj (beige) stem cells, with the characteristic beige neutrophil marker as a criterion of the takeover of host marrow by donor marrow. A hyperbolic pattern of W/WV marrow replacement conforming to a log dose-response...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184248/ https://www.ncbi.nlm.nih.gov/pubmed/349109 |
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collection | PubMed |
description | The kinetics of bone marrow replacement was studied in W/WV mice implanted with gbj/bgj (beige) stem cells, with the characteristic beige neutrophil marker as a criterion of the takeover of host marrow by donor marrow. A hyperbolic pattern of W/WV marrow replacement conforming to a log dose-response was observed in experiments encompassing a 50-fold range of bgj/bgj inoculum doses and a 2-yr period of observation. The dose-response relationships were consistent with random seeding of stem cells in the host marrow coupled with a decreasing efficiency of secondary colonization by local migration. Application of single-hit Poisson sampling statistics to the dose- response data led to the hypothesis that mouse bone marrow is compartmentalized into essentially self-contained stem cell regulatory volumes or domains. We estimate that W/WV marrow contains about 2,600 stem cells regulatory units with an average volume of about 10(8) micron3, a dimension consistent with the presumptive role of short- range cell-cell interactions in the regulation of pluripotent stem cells. Our analysis of the dose-response data is also indicative of the discontinuous and limited nature of local stem cell migration in a cellular marrow, a consideration that may be of practical as well as theoretical interest. |
format | Text |
id | pubmed-2184248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21842482008-04-17 Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera J Exp Med Articles The kinetics of bone marrow replacement was studied in W/WV mice implanted with gbj/bgj (beige) stem cells, with the characteristic beige neutrophil marker as a criterion of the takeover of host marrow by donor marrow. A hyperbolic pattern of W/WV marrow replacement conforming to a log dose-response was observed in experiments encompassing a 50-fold range of bgj/bgj inoculum doses and a 2-yr period of observation. The dose-response relationships were consistent with random seeding of stem cells in the host marrow coupled with a decreasing efficiency of secondary colonization by local migration. Application of single-hit Poisson sampling statistics to the dose- response data led to the hypothesis that mouse bone marrow is compartmentalized into essentially self-contained stem cell regulatory volumes or domains. We estimate that W/WV marrow contains about 2,600 stem cells regulatory units with an average volume of about 10(8) micron3, a dimension consistent with the presumptive role of short- range cell-cell interactions in the regulation of pluripotent stem cells. Our analysis of the dose-response data is also indicative of the discontinuous and limited nature of local stem cell migration in a cellular marrow, a consideration that may be of practical as well as theoretical interest. The Rockefeller University Press 1978-04-01 /pmc/articles/PMC2184248/ /pubmed/349109 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera |
title | Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera |
title_full | Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera |
title_fullStr | Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera |
title_full_unstemmed | Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera |
title_short | Hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:W/WV chimera |
title_sort | hematopoietic stem cell regulatory volumes as revealed in studies of the bgj/bgj:w/wv chimera |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184248/ https://www.ncbi.nlm.nih.gov/pubmed/349109 |