Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity

A method has been developed for the enrichment of the hematopoietic precursors of thymocytes from spleen and bone marrow cells. Up to 40- fold enrichments were obtained resulting in preparations in which as few as 10(5) cells produced prompt repopulation of the thymus of an irradiated mouse. Precurs...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184306/
https://www.ncbi.nlm.nih.gov/pubmed/308093
_version_ 1782145676255166464
collection PubMed
description A method has been developed for the enrichment of the hematopoietic precursors of thymocytes from spleen and bone marrow cells. Up to 40- fold enrichments were obtained resulting in preparations in which as few as 10(5) cells produced prompt repopulation of the thymus of an irradiated mouse. Precursor cells from bone marrow appear to contain the enzyme terminal deoxyribonucleotidyl transferase (Tdt), an agent suggested as a potential somatic mutator. This enzyme (Tdt) was not detectable in any spleen cell preparation examined, including one in which a 40-fold enrichment of thymocyte precursors had been produced. This is the first difference reported between the splenic and bone marrow precursors of thymocytes.
format Text
id pubmed-2184306
institution National Center for Biotechnology Information
language English
publishDate 1978
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21843062008-04-17 Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity J Exp Med Articles A method has been developed for the enrichment of the hematopoietic precursors of thymocytes from spleen and bone marrow cells. Up to 40- fold enrichments were obtained resulting in preparations in which as few as 10(5) cells produced prompt repopulation of the thymus of an irradiated mouse. Precursor cells from bone marrow appear to contain the enzyme terminal deoxyribonucleotidyl transferase (Tdt), an agent suggested as a potential somatic mutator. This enzyme (Tdt) was not detectable in any spleen cell preparation examined, including one in which a 40-fold enrichment of thymocyte precursors had been produced. This is the first difference reported between the splenic and bone marrow precursors of thymocytes. The Rockefeller University Press 1978-06-01 /pmc/articles/PMC2184306/ /pubmed/308093 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity
title Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity
title_full Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity
title_fullStr Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity
title_full_unstemmed Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity
title_short Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity
title_sort hematopoietic thymocyte precursors: iv. enrichment of the precursors and evidence for heterogeneity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184306/
https://www.ncbi.nlm.nih.gov/pubmed/308093

Ejemplares similares