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Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation

We have previously demonstrated that invasion of erythrocytes (RBCs) by malaria merozoites follows a sequence: recognition and attachment in an apical orientation associated with widespread deformation of the RBC, junction formation, movement of the junction around the merozoite that brings the mero...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1979
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184746/
https://www.ncbi.nlm.nih.gov/pubmed/105074
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description We have previously demonstrated that invasion of erythrocytes (RBCs) by malaria merozoites follows a sequence: recognition and attachment in an apical orientation associated with widespread deformation of the RBC, junction formation, movement of the junction around the merozoite that brings the merozoite into the invaginated RBC membrane, and sealing of the membrane. In the present paper, we describe a method for blocking invasion at an early stage in the sequence. Cytochalasin-treated merozoites attach specifically to host RBCs, most frequently by the apical region that contains specialized organelles (rhoptries) associated with invasion. The parasite then forms a junction between the apical region and the RBC. Cytochalasin blocks movement of this junction, a later step in invasion. Cytochalasin-treated (Plasmodium knowlesi) merozoites attach to Duffy-negative human RBCs, although these RBCs are resistant to invasion by the parasite. The attachment with these RBCs, however, differs from susceptible RBCs in that there is no junction formation. Therefore the Duffy associated antigen appears to be involved in junction formation, not initial attachment.
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spelling pubmed-21847462008-04-17 Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation J Exp Med Articles We have previously demonstrated that invasion of erythrocytes (RBCs) by malaria merozoites follows a sequence: recognition and attachment in an apical orientation associated with widespread deformation of the RBC, junction formation, movement of the junction around the merozoite that brings the merozoite into the invaginated RBC membrane, and sealing of the membrane. In the present paper, we describe a method for blocking invasion at an early stage in the sequence. Cytochalasin-treated merozoites attach specifically to host RBCs, most frequently by the apical region that contains specialized organelles (rhoptries) associated with invasion. The parasite then forms a junction between the apical region and the RBC. Cytochalasin blocks movement of this junction, a later step in invasion. Cytochalasin-treated (Plasmodium knowlesi) merozoites attach to Duffy-negative human RBCs, although these RBCs are resistant to invasion by the parasite. The attachment with these RBCs, however, differs from susceptible RBCs in that there is no junction formation. Therefore the Duffy associated antigen appears to be involved in junction formation, not initial attachment. The Rockefeller University Press 1979-01-01 /pmc/articles/PMC2184746/ /pubmed/105074 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation
title Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation
title_full Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation
title_fullStr Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation
title_full_unstemmed Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation
title_short Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation
title_sort interaction between cytochalasin b-treated malarial parasites and erythrocytes. attachment and junction formation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2184746/
https://www.ncbi.nlm.nih.gov/pubmed/105074