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Preparation of functional smooth muscle cells from the rabbit aorta
A procedure for dissociating the rabbit aorta into single, functional smooth muscle cells is described. After removal of adventitia and intima, slices of media were incubated with purified collagenase, elastase, and soybean trypsin inhibitor in a Krebs-Ringer buffer modified with Hepes, amino acids,...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185053/ https://www.ncbi.nlm.nih.gov/pubmed/214509 |