B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay

A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absol...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185108/
https://www.ncbi.nlm.nih.gov/pubmed/309925
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collection PubMed
description A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.
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spelling pubmed-21851082008-04-17 B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay J Exp Med Articles A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells. The Rockefeller University Press 1978-12-01 /pmc/articles/PMC2185108/ /pubmed/309925 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay
title B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay
title_full B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay
title_fullStr B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay
title_full_unstemmed B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay
title_short B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay
title_sort b-cell precursors specific to sheep erythrocytes. estimation of frequency in a specific helper assay
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185108/
https://www.ncbi.nlm.nih.gov/pubmed/309925

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