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The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast
The spindle position checkpoint in Saccharomyces cerevisiae delays mitotic exit until the spindle has moved into the mother–bud neck, ensuring that each daughter cell inherits a nucleus. The small G protein Tem1p is critical in promoting mitotic exit and is concentrated at the spindle pole destined...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185533/ https://www.ncbi.nlm.nih.gov/pubmed/11285282 |
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author | Adames, Neil R. Oberle, Jessica R. Cooper, John A. |
author_facet | Adames, Neil R. Oberle, Jessica R. Cooper, John A. |
author_sort | Adames, Neil R. |
collection | PubMed |
description | The spindle position checkpoint in Saccharomyces cerevisiae delays mitotic exit until the spindle has moved into the mother–bud neck, ensuring that each daughter cell inherits a nucleus. The small G protein Tem1p is critical in promoting mitotic exit and is concentrated at the spindle pole destined for the bud. The presumed nucleotide exchange factor for Tem1p, Lte1p, is concentrated in the bud. These findings suggested the hypothesis that movement of the spindle pole through the neck allows Tem1p to interact with Lte1p, promoting GTP loading of Tem1p and mitotic exit. However, we report that deletion of LTE1 had little effect on the timing of mitotic exit. We also examined several mutants in which some cells inappropriately exit mitosis even though the spindle is within the mother. In some of these cells, the spindle pole body did not interact with the bud or the neck before mitotic exit. Thus, some alternative mechanism must exist to coordinate mitotic exit with spindle position. In both wild-type and mutant cells, mitotic exit was preceded by loss of cytoplasmic microtubules from the neck. Thus, the spindle position checkpoint may monitor such interactions. |
format | Text |
id | pubmed-2185533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21855332008-05-01 The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast Adames, Neil R. Oberle, Jessica R. Cooper, John A. J Cell Biol Original Article The spindle position checkpoint in Saccharomyces cerevisiae delays mitotic exit until the spindle has moved into the mother–bud neck, ensuring that each daughter cell inherits a nucleus. The small G protein Tem1p is critical in promoting mitotic exit and is concentrated at the spindle pole destined for the bud. The presumed nucleotide exchange factor for Tem1p, Lte1p, is concentrated in the bud. These findings suggested the hypothesis that movement of the spindle pole through the neck allows Tem1p to interact with Lte1p, promoting GTP loading of Tem1p and mitotic exit. However, we report that deletion of LTE1 had little effect on the timing of mitotic exit. We also examined several mutants in which some cells inappropriately exit mitosis even though the spindle is within the mother. In some of these cells, the spindle pole body did not interact with the bud or the neck before mitotic exit. Thus, some alternative mechanism must exist to coordinate mitotic exit with spindle position. In both wild-type and mutant cells, mitotic exit was preceded by loss of cytoplasmic microtubules from the neck. Thus, the spindle position checkpoint may monitor such interactions. The Rockefeller University Press 2001-04-02 /pmc/articles/PMC2185533/ /pubmed/11285282 Text en © 2001 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Adames, Neil R. Oberle, Jessica R. Cooper, John A. The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast |
title | The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast |
title_full | The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast |
title_fullStr | The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast |
title_full_unstemmed | The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast |
title_short | The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast |
title_sort | surveillance mechanism of the spindle position checkpoint in yeast |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185533/ https://www.ncbi.nlm.nih.gov/pubmed/11285282 |
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