Cargando…
CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chr...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2000
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185570/ https://www.ncbi.nlm.nih.gov/pubmed/10893249 |
_version_ | 1782145770889150464 |
---|---|
author | Yucel, Jennifer K. Marszalek, Janet D. McIntosh, J. Richard Goldstein, Lawrence S.B. Cleveland, Don W. Philp, Alastair Valentine |
author_facet | Yucel, Jennifer K. Marszalek, Janet D. McIntosh, J. Richard Goldstein, Lawrence S.B. Cleveland, Don W. Philp, Alastair Valentine |
author_sort | Yucel, Jennifer K. |
collection | PubMed |
description | CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements. |
format | Text |
id | pubmed-2185570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21855702008-05-01 CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila Yucel, Jennifer K. Marszalek, Janet D. McIntosh, J. Richard Goldstein, Lawrence S.B. Cleveland, Don W. Philp, Alastair Valentine J Cell Biol Original Article CENP-meta has been identified as an essential, kinesin-like motor protein in Drosophila. The 257-kD CENP-meta protein is most similar to the vertebrate kinetochore-associated kinesin-like protein CENP-E, and like CENP-E, is shown to be a component of centromeric/kinetochore regions of Drosophila chromosomes. However, unlike CENP-E, which leaves the centromere/kinetochore region at the end of anaphase A, the CENP-meta protein remains associated with the centromeric/kinetochore region of the chromosome during all stages of the Drosophila cell cycle. P-element–mediated disruption of the CENP-meta gene leads to late larval/pupal stage lethality with incomplete chromosome alignment at metaphase. Complete removal of CENP-meta from the female germline leads to lethality in early embryos resulting from defects in metaphase chromosome alignment. Real-time imaging of these mutants with GFP-labeled chromosomes demonstrates that CENP-meta is required for the maintenance of chromosomes at the metaphase plate, demonstrating that the functions required to establish and maintain chromosome congression have distinguishable requirements. The Rockefeller University Press 2000-07-10 /pmc/articles/PMC2185570/ /pubmed/10893249 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Yucel, Jennifer K. Marszalek, Janet D. McIntosh, J. Richard Goldstein, Lawrence S.B. Cleveland, Don W. Philp, Alastair Valentine CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila |
title |
CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
|
title_full |
CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
|
title_fullStr |
CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
|
title_full_unstemmed |
CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
|
title_short |
CENP-meta, an Essential Kinetochore Kinesin Required for the Maintenance of Metaphase Chromosome Alignment in Drosophila
|
title_sort | cenp-meta, an essential kinetochore kinesin required for the maintenance of metaphase chromosome alignment in drosophila |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185570/ https://www.ncbi.nlm.nih.gov/pubmed/10893249 |
work_keys_str_mv | AT yuceljenniferk cenpmetaanessentialkinetochorekinesinrequiredforthemaintenanceofmetaphasechromosomealignmentindrosophila AT marszalekjanetd cenpmetaanessentialkinetochorekinesinrequiredforthemaintenanceofmetaphasechromosomealignmentindrosophila AT mcintoshjrichard cenpmetaanessentialkinetochorekinesinrequiredforthemaintenanceofmetaphasechromosomealignmentindrosophila AT goldsteinlawrencesb cenpmetaanessentialkinetochorekinesinrequiredforthemaintenanceofmetaphasechromosomealignmentindrosophila AT clevelanddonw cenpmetaanessentialkinetochorekinesinrequiredforthemaintenanceofmetaphasechromosomealignmentindrosophila AT philpalastairvalentine cenpmetaanessentialkinetochorekinesinrequiredforthemaintenanceofmetaphasechromosomealignmentindrosophila |