Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally

Lipopolysaccharide (LPS)-activated B-cell blasts from C57BL/6J nu/nu spleen cells develop into IgM-secreting clones after stimulation by antigen-specific T-helper cells of C57BL/6J origin. Although induction of help is antigen-dependent, help itself acts polyclonally. 1 of 1--3 B-cell blasts is rest...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1980
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185768/
https://www.ncbi.nlm.nih.gov/pubmed/6965306
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description Lipopolysaccharide (LPS)-activated B-cell blasts from C57BL/6J nu/nu spleen cells develop into IgM-secreting clones after stimulation by antigen-specific T-helper cells of C57BL/6J origin. Although induction of help is antigen-dependent, help itself acts polyclonally. 1 of 1--3 B-cell blasts is restimulated in a homologous fashion by LPS, or in a heterologous fashion by sheep erythrocyte (SRC)- or horse erythrocyte (HRC)-activated T-helper cells. The repertoire of activated B-cell blasts reflects the polyclonal nature of activation: approximately 1 in 1,000--3,000 restimulated B-cell blasts is specific for SRC, 1 in 300-- 1,000 is specific for HRC, and 1 in 100--300 specific for trinitrophenylated SRC (TNP30-SRC). B-cell blasts that are either H-2 compatible or H-2 incompatible with the antigen-activated T-cell help are stimulated polyclonally in similar high frequencies. Thus, neither antigen nor H-2 compatibility are required to stimulate a B-cell blast into the next cell cycle.
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spelling pubmed-21857682008-04-17 Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally J Exp Med Articles Lipopolysaccharide (LPS)-activated B-cell blasts from C57BL/6J nu/nu spleen cells develop into IgM-secreting clones after stimulation by antigen-specific T-helper cells of C57BL/6J origin. Although induction of help is antigen-dependent, help itself acts polyclonally. 1 of 1--3 B-cell blasts is restimulated in a homologous fashion by LPS, or in a heterologous fashion by sheep erythrocyte (SRC)- or horse erythrocyte (HRC)-activated T-helper cells. The repertoire of activated B-cell blasts reflects the polyclonal nature of activation: approximately 1 in 1,000--3,000 restimulated B-cell blasts is specific for SRC, 1 in 300-- 1,000 is specific for HRC, and 1 in 100--300 specific for trinitrophenylated SRC (TNP30-SRC). B-cell blasts that are either H-2 compatible or H-2 incompatible with the antigen-activated T-cell help are stimulated polyclonally in similar high frequencies. Thus, neither antigen nor H-2 compatibility are required to stimulate a B-cell blast into the next cell cycle. The Rockefeller University Press 1980-01-01 /pmc/articles/PMC2185768/ /pubmed/6965306 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally
title Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally
title_full Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally
title_fullStr Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally
title_full_unstemmed Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally
title_short Antigen-specific T-helper cells stimulate H-2-compatible and H-2- incompatible B-cell blasts polyclonally
title_sort antigen-specific t-helper cells stimulate h-2-compatible and h-2- incompatible b-cell blasts polyclonally
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185768/
https://www.ncbi.nlm.nih.gov/pubmed/6965306

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