Cargando…
Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes
Blood form trypomastigotes of the Y and CL strains of Trypanosoma cruzi were tested for their ability to enter and infect mouse peritoneal macrophages. Both strains failed to enter macrophages in appreciable numbers, whereas metacyclic trypomastigotes purified from acellular cultures were ingested w...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1980
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185941/ https://www.ncbi.nlm.nih.gov/pubmed/6995556 |
_version_ | 1782145854116724736 |
---|---|
collection | PubMed |
description | Blood form trypomastigotes of the Y and CL strains of Trypanosoma cruzi were tested for their ability to enter and infect mouse peritoneal macrophages. Both strains failed to enter macrophages in appreciable numbers, whereas metacyclic trypomastigotes purified from acellular cultures were ingested with ease. Macrophage parasitization was enhanced manyfold after the removal of an antiphagocytic substance by trypsinization. This occurred without modification of parasite viability. Opsonization with hyperimmune mouse serum also enhanced the uptake of blood form trypomastigotes by macrophages. This effect was mediated by the macrophage Fc receptor. The effects of serum and trypsinization were additive at high parasite:cell ratios. Neither trypsin-mediated nor antibody-dependent opsonization of the organisms modified the fate of either strain within resident macrophages. However, lymphokine-activated macrophages were capable of destroying both strains, and antibody opsonization further enhanced this process. |
format | Text |
id | pubmed-2185941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21859412008-04-17 Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes J Exp Med Articles Blood form trypomastigotes of the Y and CL strains of Trypanosoma cruzi were tested for their ability to enter and infect mouse peritoneal macrophages. Both strains failed to enter macrophages in appreciable numbers, whereas metacyclic trypomastigotes purified from acellular cultures were ingested with ease. Macrophage parasitization was enhanced manyfold after the removal of an antiphagocytic substance by trypsinization. This occurred without modification of parasite viability. Opsonization with hyperimmune mouse serum also enhanced the uptake of blood form trypomastigotes by macrophages. This effect was mediated by the macrophage Fc receptor. The effects of serum and trypsinization were additive at high parasite:cell ratios. Neither trypsin-mediated nor antibody-dependent opsonization of the organisms modified the fate of either strain within resident macrophages. However, lymphokine-activated macrophages were capable of destroying both strains, and antibody opsonization further enhanced this process. The Rockefeller University Press 1980-08-01 /pmc/articles/PMC2185941/ /pubmed/6995556 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes |
title | Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes |
title_full | Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes |
title_fullStr | Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes |
title_full_unstemmed | Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes |
title_short | Trypanosoma cruzi. Factors modifying ingestion and fate of blood form trypomastigotes |
title_sort | trypanosoma cruzi. factors modifying ingestion and fate of blood form trypomastigotes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185941/ https://www.ncbi.nlm.nih.gov/pubmed/6995556 |