Growth of factor-dependent hemopoietic precursor cell lines

Cell lines have been produced from long-term cultures of mouse bone marrow that require a factor, present in WEHI-3 conditioned medium (CM) or in spleen CM, for their sustained growth. The cell lines were obtained from nonvirus-treated cultures, are nonleukemic, maintain a normal karyotype, and form...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1980
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185980/
https://www.ncbi.nlm.nih.gov/pubmed/6968334
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description Cell lines have been produced from long-term cultures of mouse bone marrow that require a factor, present in WEHI-3 conditioned medium (CM) or in spleen CM, for their sustained growth. The cell lines were obtained from nonvirus-treated cultures, are nonleukemic, maintain a normal karyotype, and form colonies showing granulocyte maturation when plated in soft agar. Granulocyte/macrophage (GM) colony-stimulating factor is not the inductive moiety involved in the maintenance of proliferation of these cells. It is suggested that the cell lines represent a self-renewing population of cells ancestral to GM colony- forming cells, which may be responding to a hitherto unrecognized regulator.
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spelling pubmed-21859802008-04-17 Growth of factor-dependent hemopoietic precursor cell lines J Exp Med Articles Cell lines have been produced from long-term cultures of mouse bone marrow that require a factor, present in WEHI-3 conditioned medium (CM) or in spleen CM, for their sustained growth. The cell lines were obtained from nonvirus-treated cultures, are nonleukemic, maintain a normal karyotype, and form colonies showing granulocyte maturation when plated in soft agar. Granulocyte/macrophage (GM) colony-stimulating factor is not the inductive moiety involved in the maintenance of proliferation of these cells. It is suggested that the cell lines represent a self-renewing population of cells ancestral to GM colony- forming cells, which may be responding to a hitherto unrecognized regulator. The Rockefeller University Press 1980-10-01 /pmc/articles/PMC2185980/ /pubmed/6968334 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Growth of factor-dependent hemopoietic precursor cell lines
title Growth of factor-dependent hemopoietic precursor cell lines
title_full Growth of factor-dependent hemopoietic precursor cell lines
title_fullStr Growth of factor-dependent hemopoietic precursor cell lines
title_full_unstemmed Growth of factor-dependent hemopoietic precursor cell lines
title_short Growth of factor-dependent hemopoietic precursor cell lines
title_sort growth of factor-dependent hemopoietic precursor cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2185980/
https://www.ncbi.nlm.nih.gov/pubmed/6968334

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