Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture

Mixed lymphocyte reaction (MLR)-activated T cells were analyzed according to the expression of various cell surface markers by the specific cytotoxic T lymphocytes (CTL) generated in the MLR. CTL were found exclusively in a population of MLR-activated T cells that lacked detectable Fc gamma R but th...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1981
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186048/
https://www.ncbi.nlm.nih.gov/pubmed/6450261
_version_ 1782145878880944128
collection PubMed
description Mixed lymphocyte reaction (MLR)-activated T cells were analyzed according to the expression of various cell surface markers by the specific cytotoxic T lymphocytes (CTL) generated in the MLR. CTL were found exclusively in a population of MLR-activated T cells that lacked detectable Fc gamma R but that expressed a surface antigen recognized by the 4F2 monoclonal antibody. In contrast, CTL were found in both the Ia-positive and Ia-negative cells after MLR activation. Thus, the specific CTL generated in the allogeneic MLR can be identified and isolated by virtue of the expression of a particular cell surface marker.
format Text
id pubmed-2186048
institution National Center for Biotechnology Information
language English
publishDate 1981
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21860482008-04-17 Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture J Exp Med Articles Mixed lymphocyte reaction (MLR)-activated T cells were analyzed according to the expression of various cell surface markers by the specific cytotoxic T lymphocytes (CTL) generated in the MLR. CTL were found exclusively in a population of MLR-activated T cells that lacked detectable Fc gamma R but that expressed a surface antigen recognized by the 4F2 monoclonal antibody. In contrast, CTL were found in both the Ia-positive and Ia-negative cells after MLR activation. Thus, the specific CTL generated in the allogeneic MLR can be identified and isolated by virtue of the expression of a particular cell surface marker. The Rockefeller University Press 1981-01-01 /pmc/articles/PMC2186048/ /pubmed/6450261 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture
title Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture
title_full Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture
title_fullStr Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture
title_full_unstemmed Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture
title_short Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture
title_sort phenotypic characterization of human cytolytic t lymphocytes in mixed lymphocyte culture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186048/
https://www.ncbi.nlm.nih.gov/pubmed/6450261

Ejemplares similares