Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes

Preincubation for 10-30 min with trypsin, pronase, chymotrypsin, or papain primed macrophages to undergo a twofold to sixfold increase in oxidative metabolism, measured as release of superoxide anion or hydrogen peroxide, during stimulation by phorbol myristate acetate or ingestion of Candida paraps...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1981
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186182/
https://www.ncbi.nlm.nih.gov/pubmed/6265588
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description Preincubation for 10-30 min with trypsin, pronase, chymotrypsin, or papain primed macrophages to undergo a twofold to sixfold increase in oxidative metabolism, measured as release of superoxide anion or hydrogen peroxide, during stimulation by phorbol myristate acetate or ingestion of Candida parapsilosis. Preincubation of macrophages with inactivated proteases, nonenzyme proteins, or neuraminidase did not affect their oxidase response. Exposure of macrophages to proteases generated at sites of inflammation could prime these cells for a more effective oxidase response to phagocytosis or for greater tissue damage from release of toxic oxygen metabolites.
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spelling pubmed-21861822008-04-17 Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes J Exp Med Articles Preincubation for 10-30 min with trypsin, pronase, chymotrypsin, or papain primed macrophages to undergo a twofold to sixfold increase in oxidative metabolism, measured as release of superoxide anion or hydrogen peroxide, during stimulation by phorbol myristate acetate or ingestion of Candida parapsilosis. Preincubation of macrophages with inactivated proteases, nonenzyme proteins, or neuraminidase did not affect their oxidase response. Exposure of macrophages to proteases generated at sites of inflammation could prime these cells for a more effective oxidase response to phagocytosis or for greater tissue damage from release of toxic oxygen metabolites. The Rockefeller University Press 1981-06-01 /pmc/articles/PMC2186182/ /pubmed/6265588 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
title Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
title_full Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
title_fullStr Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
title_full_unstemmed Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
title_short Priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
title_sort priming of macrophages for enhanced oxidative metabolism by exposure to proteolytic enzymes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186182/
https://www.ncbi.nlm.nih.gov/pubmed/6265588

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