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Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis

Asymmetrical growth of the vertebrae has been implicated as one possible etiologic factor in the pathogenesis of adolescent idiopathic scoliosis. The longitudinal vertebral growth derives from the endochondral ossification of the vertebral growth plate. In the present study, the growth plates from t...

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Autores principales: Wang, Shoufeng, Qiu, Yong, Zhu, Zezhang, Ma, Zhaolong, Xia, Caiwei, Zhu, Feng
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186319/
https://www.ncbi.nlm.nih.gov/pubmed/17996118
http://dx.doi.org/10.1186/1749-799X-2-19
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author Wang, Shoufeng
Qiu, Yong
Zhu, Zezhang
Ma, Zhaolong
Xia, Caiwei
Zhu, Feng
author_facet Wang, Shoufeng
Qiu, Yong
Zhu, Zezhang
Ma, Zhaolong
Xia, Caiwei
Zhu, Feng
author_sort Wang, Shoufeng
collection PubMed
description Asymmetrical growth of the vertebrae has been implicated as one possible etiologic factor in the pathogenesis of adolescent idiopathic scoliosis. The longitudinal vertebral growth derives from the endochondral ossification of the vertebral growth plate. In the present study, the growth plates from the convex and concave side of the vertebrae were characterized by the method of histology and immunohistochemistry to evaluate the growth activity, cell proliferation, and apoptosis. Normal zoned architectures were observed in the convex side of the growth plate and disorganized architectures in the concave side. The histological grades were significantly different between the convex and the concave side of the growth plate in the apex vertebrae (P < 0.05). The histological difference was also found significant statistically between end vertebrae and apex vertebrae in the concave side of vertebral growth plates (P < 0.05). The proliferative potential indexes and apoptosis indexes of chondrocytes in the proliferative and hypertrophic zone in the convex side were significantly higher than that in the concave side in the apex vertebral growth plate (P < 0.05). There was a significant difference of the proliferative potential index (proliferating cell nuclear antigen, PCNA index) between convex side and concave side at the upper end vertebra (P < 0.05). The difference of the proliferative potential index and apoptosis index were found significant statistically in the concave side of the vertebral growth plate between end vertebrae and apex vertebrae (P < 0.05). The same result was also found for the apoptosis index (terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labeling assay, TUNEL index) in the convex side of vertebral growth plate between end vertebrae and apex vertebrae (P < 0.05). Some correlation were found between radiographic measurements and proliferation and apoptosis indexes. The difference in histological grades and cellular activity between the convex and concave side indicated that the bilateral growth plate of the vertebrae in AIS patients have different growth kinetics which may affect the curve progression.
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spelling pubmed-21863192008-01-10 Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis Wang, Shoufeng Qiu, Yong Zhu, Zezhang Ma, Zhaolong Xia, Caiwei Zhu, Feng J Orthop Surg Research Article Asymmetrical growth of the vertebrae has been implicated as one possible etiologic factor in the pathogenesis of adolescent idiopathic scoliosis. The longitudinal vertebral growth derives from the endochondral ossification of the vertebral growth plate. In the present study, the growth plates from the convex and concave side of the vertebrae were characterized by the method of histology and immunohistochemistry to evaluate the growth activity, cell proliferation, and apoptosis. Normal zoned architectures were observed in the convex side of the growth plate and disorganized architectures in the concave side. The histological grades were significantly different between the convex and the concave side of the growth plate in the apex vertebrae (P < 0.05). The histological difference was also found significant statistically between end vertebrae and apex vertebrae in the concave side of vertebral growth plates (P < 0.05). The proliferative potential indexes and apoptosis indexes of chondrocytes in the proliferative and hypertrophic zone in the convex side were significantly higher than that in the concave side in the apex vertebral growth plate (P < 0.05). There was a significant difference of the proliferative potential index (proliferating cell nuclear antigen, PCNA index) between convex side and concave side at the upper end vertebra (P < 0.05). The difference of the proliferative potential index and apoptosis index were found significant statistically in the concave side of the vertebral growth plate between end vertebrae and apex vertebrae (P < 0.05). The same result was also found for the apoptosis index (terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labeling assay, TUNEL index) in the convex side of vertebral growth plate between end vertebrae and apex vertebrae (P < 0.05). Some correlation were found between radiographic measurements and proliferation and apoptosis indexes. The difference in histological grades and cellular activity between the convex and concave side indicated that the bilateral growth plate of the vertebrae in AIS patients have different growth kinetics which may affect the curve progression. BioMed Central 2007-11-11 /pmc/articles/PMC2186319/ /pubmed/17996118 http://dx.doi.org/10.1186/1749-799X-2-19 Text en Copyright © 2007 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Shoufeng
Qiu, Yong
Zhu, Zezhang
Ma, Zhaolong
Xia, Caiwei
Zhu, Feng
Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
title Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
title_full Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
title_fullStr Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
title_full_unstemmed Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
title_short Histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
title_sort histomorphological study of the spinal growth plates from the convex side and the concave side in adolescent idiopathic scoliosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186319/
https://www.ncbi.nlm.nih.gov/pubmed/17996118
http://dx.doi.org/10.1186/1749-799X-2-19
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