Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle

We have investigated mitotic cell cycle and growth phase regulation of homogeneous cytolytic T lymphocytes (CTL). Two independently derived CTL clones were stained with the DNA-binding dye Hoechst 33342, sorted in a fluorescence-activated cell sorter according to their position in the cell cycle, an...

Descripción completa

Detalles Bibliográficos
Autores principales: Sekaly, RP, MacDonald, HR, Zaech, P, Glasebrook, AL, Cerottini, J-C
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1981
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186426/
https://www.ncbi.nlm.nih.gov/pubmed/6973610
_version_ 1782145938703253504
author Sekaly, RP
MacDonald, HR
Zaech, P
Glasebrook, AL
Cerottini, J-C
author_facet Sekaly, RP
MacDonald, HR
Zaech, P
Glasebrook, AL
Cerottini, J-C
author_sort Sekaly, RP
collection PubMed
description We have investigated mitotic cell cycle and growth phase regulation of homogeneous cytolytic T lymphocytes (CTL). Two independently derived CTL clones were stained with the DNA-binding dye Hoechst 33342, sorted in a fluorescence-activated cell sorter according to their position in the cell cycle, and then assayed for specific lytic activity using a short-term (30 min) (51)Cr release assay. Results show that lytic activity remained unchanged throughout the cell cycle. Furthermore, there was no significant difference in the lytic activity of CTL clones growing exponentially or arrested in a plateau phase. These results demonstrate that T cell-mediated cytolysis is independent of growth phase and position in the cell cycle.
format Text
id pubmed-2186426
institution National Center for Biotechnology Information
language English
publishDate 1981
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21864262008-04-17 Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle Sekaly, RP MacDonald, HR Zaech, P Glasebrook, AL Cerottini, J-C J Exp Med Articles We have investigated mitotic cell cycle and growth phase regulation of homogeneous cytolytic T lymphocytes (CTL). Two independently derived CTL clones were stained with the DNA-binding dye Hoechst 33342, sorted in a fluorescence-activated cell sorter according to their position in the cell cycle, and then assayed for specific lytic activity using a short-term (30 min) (51)Cr release assay. Results show that lytic activity remained unchanged throughout the cell cycle. Furthermore, there was no significant difference in the lytic activity of CTL clones growing exponentially or arrested in a plateau phase. These results demonstrate that T cell-mediated cytolysis is independent of growth phase and position in the cell cycle. The Rockefeller University Press 1981-08-01 /pmc/articles/PMC2186426/ /pubmed/6973610 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Sekaly, RP
MacDonald, HR
Zaech, P
Glasebrook, AL
Cerottini, J-C
Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle
title Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle
title_full Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle
title_fullStr Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle
title_full_unstemmed Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle
title_short Cytolytic T lymphocyte function is independent of growth phase and position in the mitotic cycle
title_sort cytolytic t lymphocyte function is independent of growth phase and position in the mitotic cycle
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186426/
https://www.ncbi.nlm.nih.gov/pubmed/6973610
work_keys_str_mv AT sekalyrp cytolytictlymphocytefunctionisindependentofgrowthphaseandpositioninthemitoticcycle
AT macdonaldhr cytolytictlymphocytefunctionisindependentofgrowthphaseandpositioninthemitoticcycle
AT zaechp cytolytictlymphocytefunctionisindependentofgrowthphaseandpositioninthemitoticcycle
AT glasebrookal cytolytictlymphocytefunctionisindependentofgrowthphaseandpositioninthemitoticcycle
AT cerottinijc cytolytictlymphocytefunctionisindependentofgrowthphaseandpositioninthemitoticcycle

Ejemplares similares