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Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors
Five hybridoma T cell lines were prepared by fusion of second order suppressor T cells (Ts2) with the BW5147 thymoma. The culture supernates from these T cell hybrids contained a factor, TsF2, which specifically suppressed 4-hydroxy-3-nitrophenyl acetyl hapten (NP)- induced cutaneous sensitivity res...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1981
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186527/ https://www.ncbi.nlm.nih.gov/pubmed/6170719 |
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collection | PubMed |
description | Five hybridoma T cell lines were prepared by fusion of second order suppressor T cells (Ts2) with the BW5147 thymoma. The culture supernates from these T cell hybrids contained a factor, TsF2, which specifically suppressed 4-hydroxy-3-nitrophenyl acetyl hapten (NP)- induced cutaneous sensitivity responses. TsF2 activity was observed when the factor was administered during the effector phases of the immune response. TsF2 bears I-J determinants and has binding specificity for NPb idiotypic determinants. TsF2 suppressor activity could be absorbed on antigen-primed H-2-incompatible T cells but cannot suppress H-2-incompatible mice. In addition to this H-2 restriction, which maps to the I-J subregion, monoclonal TsF2 also has an Igh genetic restriction. The present results are combined with previous data to describe the cellular interactions leading to immune suppression. |
format | Text |
id | pubmed-2186527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1981 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21865272008-04-17 Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors J Exp Med Articles Five hybridoma T cell lines were prepared by fusion of second order suppressor T cells (Ts2) with the BW5147 thymoma. The culture supernates from these T cell hybrids contained a factor, TsF2, which specifically suppressed 4-hydroxy-3-nitrophenyl acetyl hapten (NP)- induced cutaneous sensitivity responses. TsF2 activity was observed when the factor was administered during the effector phases of the immune response. TsF2 bears I-J determinants and has binding specificity for NPb idiotypic determinants. TsF2 suppressor activity could be absorbed on antigen-primed H-2-incompatible T cells but cannot suppress H-2-incompatible mice. In addition to this H-2 restriction, which maps to the I-J subregion, monoclonal TsF2 also has an Igh genetic restriction. The present results are combined with previous data to describe the cellular interactions leading to immune suppression. The Rockefeller University Press 1981-11-01 /pmc/articles/PMC2186527/ /pubmed/6170719 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors |
title | Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors |
title_full | Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors |
title_fullStr | Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors |
title_full_unstemmed | Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors |
title_short | Analysis of T cell hybridomas. I. Characterization of H-2 and Igh- restricted monoclonal suppressor factors |
title_sort | analysis of t cell hybridomas. i. characterization of h-2 and igh- restricted monoclonal suppressor factors |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186527/ https://www.ncbi.nlm.nih.gov/pubmed/6170719 |