Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments

The appearance of immunologically competent cells in the organ-cultured mouse fetal thymic rudiment has been investigated. Fetal thymuses removed at 14 d of gestation and cultured for 7-21 d were assayed for their content of cytolytic T lymphocyte precursors (CTL-P) directed against H-2d alloantigen...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186602/
https://www.ncbi.nlm.nih.gov/pubmed/6977011
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collection PubMed
description The appearance of immunologically competent cells in the organ-cultured mouse fetal thymic rudiment has been investigated. Fetal thymuses removed at 14 d of gestation and cultured for 7-21 d were assayed for their content of cytolytic T lymphocyte precursors (CTL-P) directed against H-2d alloantigens. Whereas CTL-P were undetectable within fetal thymus until 18-19 d of gestation, their frequency in the organ- cultured fetal thymus was similar to, or greater than that found in the normal adult thymus. This direct demonstration of the appearance of alloreactive CTL-P in a closed in vitro system should provide an accessible model for the investigation of interactions between developing T cells and the thymic microenvironment.
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spelling pubmed-21866022008-04-17 Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments J Exp Med Articles The appearance of immunologically competent cells in the organ-cultured mouse fetal thymic rudiment has been investigated. Fetal thymuses removed at 14 d of gestation and cultured for 7-21 d were assayed for their content of cytolytic T lymphocyte precursors (CTL-P) directed against H-2d alloantigens. Whereas CTL-P were undetectable within fetal thymus until 18-19 d of gestation, their frequency in the organ- cultured fetal thymus was similar to, or greater than that found in the normal adult thymus. This direct demonstration of the appearance of alloreactive CTL-P in a closed in vitro system should provide an accessible model for the investigation of interactions between developing T cells and the thymic microenvironment. The Rockefeller University Press 1982-02-01 /pmc/articles/PMC2186602/ /pubmed/6977011 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
title Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
title_full Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
title_fullStr Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
title_full_unstemmed Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
title_short Development of cytolytic T lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
title_sort development of cytolytic t lymphocyte precursors in organ-cultured mouse embryonic thymus rudiments
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186602/
https://www.ncbi.nlm.nih.gov/pubmed/6977011

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