Cargando…

Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones

Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cel...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186651/
https://www.ncbi.nlm.nih.gov/pubmed/6174671
_version_ 1782145991564066816
collection PubMed
description Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in our assay, or their effect may be produced by anti- idiotype. Thus, anti-idiotype may provide two or more specific T cell signals.
format Text
id pubmed-2186651
institution National Center for Biotechnology Information
language English
publishDate 1982
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21866512008-04-17 Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones J Exp Med Articles Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in our assay, or their effect may be produced by anti- idiotype. Thus, anti-idiotype may provide two or more specific T cell signals. The Rockefeller University Press 1982-04-01 /pmc/articles/PMC2186651/ /pubmed/6174671 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones
title Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones
title_full Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones
title_fullStr Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones
title_full_unstemmed Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones
title_short Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones
title_sort definition of t cell idiotypes using anti-idiotypic antisera produced by immunization with t cell clones
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186651/
https://www.ncbi.nlm.nih.gov/pubmed/6174671