A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells

Expression of VH-coded mRNA molecules in T cells, antigen-specific T cell lines, or T cell hybridomas was not detected using four different VH DNA probes under conditions that permitted cross-hybridization between distantly related VH genes. In contrast, VH gene expression was readily detected in tw...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186877/
https://www.ncbi.nlm.nih.gov/pubmed/7175441
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collection PubMed
description Expression of VH-coded mRNA molecules in T cells, antigen-specific T cell lines, or T cell hybridomas was not detected using four different VH DNA probes under conditions that permitted cross-hybridization between distantly related VH genes. In contrast, VH gene expression was readily detected in two B cell lymphomas and in splenic B cells. Less than one molecule per cell of RNA, exactly complementary to the DNA probes used, would have been detected in these T cell populations. The results thus seriously question the proposition that T cells use the B cell VH repertoire to code for antigen receptors.
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spelling pubmed-21868772008-04-17 A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells J Exp Med Articles Expression of VH-coded mRNA molecules in T cells, antigen-specific T cell lines, or T cell hybridomas was not detected using four different VH DNA probes under conditions that permitted cross-hybridization between distantly related VH genes. In contrast, VH gene expression was readily detected in two B cell lymphomas and in splenic B cells. Less than one molecule per cell of RNA, exactly complementary to the DNA probes used, would have been detected in these T cell populations. The results thus seriously question the proposition that T cells use the B cell VH repertoire to code for antigen receptors. The Rockefeller University Press 1982-12-01 /pmc/articles/PMC2186877/ /pubmed/7175441 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells
title A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells
title_full A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells
title_fullStr A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells
title_full_unstemmed A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells
title_short A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells
title_sort search for messenger rna molecules bearing immunoglobulin vh nucleotide sequences in t cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186877/
https://www.ncbi.nlm.nih.gov/pubmed/7175441

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