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Two independent receptors allow selective target lysis by T cell clones

Dimethylbenzanthracene-induced P1 sarcoma cells induce P1-specific antibodies in syngeneic DA rats. Antiidiotypic antibodies of specificity DA anti-(DA anti-P1) were induced against the tumor- specific antibodies and used to restimulate P1-primed DA T cells in vitro. Using antiidiotypic antibodies a...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186977/
https://www.ncbi.nlm.nih.gov/pubmed/6187887
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description Dimethylbenzanthracene-induced P1 sarcoma cells induce P1-specific antibodies in syngeneic DA rats. Antiidiotypic antibodies of specificity DA anti-(DA anti-P1) were induced against the tumor- specific antibodies and used to restimulate P1-primed DA T cells in vitro. Using antiidiotypic antibodies and T cell growth factor, P1- specific cytotoxic DA T cell clones were established by limiting dilution and kept in vitro. Two of these clones acquired during culture periods in addition to the P1 specificity lytic activity towards natural killer (NK) targets YAC-1 or K562. Cold target inhibition experiments showed that the very same cytotoxic T cells kill P1 and NK targets. Antiidiotypic antibodies of specificity DA anti-(DA anti-P1) inhibited cytotoxicity against P1 but not against YAC-1 or K562. We conclude that two independent receptors are located on these double- reactive T cell clones, one that is idiotypic and antigen-specific, and another displaying the binding profile of NK cells.
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spelling pubmed-21869772008-04-17 Two independent receptors allow selective target lysis by T cell clones J Exp Med Articles Dimethylbenzanthracene-induced P1 sarcoma cells induce P1-specific antibodies in syngeneic DA rats. Antiidiotypic antibodies of specificity DA anti-(DA anti-P1) were induced against the tumor- specific antibodies and used to restimulate P1-primed DA T cells in vitro. Using antiidiotypic antibodies and T cell growth factor, P1- specific cytotoxic DA T cell clones were established by limiting dilution and kept in vitro. Two of these clones acquired during culture periods in addition to the P1 specificity lytic activity towards natural killer (NK) targets YAC-1 or K562. Cold target inhibition experiments showed that the very same cytotoxic T cells kill P1 and NK targets. Antiidiotypic antibodies of specificity DA anti-(DA anti-P1) inhibited cytotoxicity against P1 but not against YAC-1 or K562. We conclude that two independent receptors are located on these double- reactive T cell clones, one that is idiotypic and antigen-specific, and another displaying the binding profile of NK cells. The Rockefeller University Press 1983-04-01 /pmc/articles/PMC2186977/ /pubmed/6187887 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Two independent receptors allow selective target lysis by T cell clones
title Two independent receptors allow selective target lysis by T cell clones
title_full Two independent receptors allow selective target lysis by T cell clones
title_fullStr Two independent receptors allow selective target lysis by T cell clones
title_full_unstemmed Two independent receptors allow selective target lysis by T cell clones
title_short Two independent receptors allow selective target lysis by T cell clones
title_sort two independent receptors allow selective target lysis by t cell clones
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186977/
https://www.ncbi.nlm.nih.gov/pubmed/6187887