Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat

A new assay system is described for the enumeration of antigen-specific IgE immunoglobulin-secreting cells (ISC) based on an enzyme-linked immunoabsorbent assay. Using this technique to monitor the organ distribution of OVA-specific ISC after primary immunization of rats, approximately 12,000 specif...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187046/
https://www.ncbi.nlm.nih.gov/pubmed/6189957
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collection PubMed
description A new assay system is described for the enumeration of antigen-specific IgE immunoglobulin-secreting cells (ISC) based on an enzyme-linked immunoabsorbent assay. Using this technique to monitor the organ distribution of OVA-specific ISC after primary immunization of rats, approximately 12,000 specific IgE ISC were detected at the peak of the response in the draining lymph nodes compared with 117,000 IgG ISC; the splenic anti-OVA response was restricted to the IgM class. Using plates precoated with anti-rat IgE instead of antigen, total IgE ISC were enumerated in normal and helminth-parasitized rats. The assay system detected up to 5 X 10(5) IgE ISC in mesenteric lymph nodes from parasitized animals compared with less than 50 in controls.
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spelling pubmed-21870462008-04-17 Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat J Exp Med Articles A new assay system is described for the enumeration of antigen-specific IgE immunoglobulin-secreting cells (ISC) based on an enzyme-linked immunoabsorbent assay. Using this technique to monitor the organ distribution of OVA-specific ISC after primary immunization of rats, approximately 12,000 specific IgE ISC were detected at the peak of the response in the draining lymph nodes compared with 117,000 IgG ISC; the splenic anti-OVA response was restricted to the IgM class. Using plates precoated with anti-rat IgE instead of antigen, total IgE ISC were enumerated in normal and helminth-parasitized rats. The assay system detected up to 5 X 10(5) IgE ISC in mesenteric lymph nodes from parasitized animals compared with less than 50 in controls. The Rockefeller University Press 1983-06-01 /pmc/articles/PMC2187046/ /pubmed/6189957 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
title Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
title_full Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
title_fullStr Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
title_full_unstemmed Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
title_short Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat
title_sort kinetics and distribution of antigen-specific ige-secreting cells during the primary antibody response in the rat
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187046/
https://www.ncbi.nlm.nih.gov/pubmed/6189957

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