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Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency

The immunodeficiency in CBA/N mice is reflected by abnormal development of a subset of B lymphocytes. However, it is not clear how xid, the mutant gene in CBA/N mice, affects the development of this subset. Specifically, it is not known if the xid gene influences the development of the B cell subset...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187116/
https://www.ncbi.nlm.nih.gov/pubmed/6604128
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description The immunodeficiency in CBA/N mice is reflected by abnormal development of a subset of B lymphocytes. However, it is not clear how xid, the mutant gene in CBA/N mice, affects the development of this subset. Specifically, it is not known if the xid gene influences the development of the B cell subset directly or indirectly by providing the improper developmental milieu through effects on other cells. We investigated this question using female mice heterozygous for two x chromosomal genes, xid and Pgk-1 (phosphoglycerate kinase-1). Since females are mosaic because of x chromosome inactivation, their lymphocytes can be studied for the choice of the x chromosome, using the two PGK-1 isoenzymes as the cytological marker. We find that B lymphocytes in the spleen prefer the x chromosome without xid while the remaining splenocytes and cells from other tissues do not. This suggests that xid affects B lymphocytes directly and not through their developmental milieu. Furthermore, our data suggest that the precursors for IgG1- and IgG3-producing cells may be both few and different.
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spelling pubmed-21871162008-04-17 Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency J Exp Med Articles The immunodeficiency in CBA/N mice is reflected by abnormal development of a subset of B lymphocytes. However, it is not clear how xid, the mutant gene in CBA/N mice, affects the development of this subset. Specifically, it is not known if the xid gene influences the development of the B cell subset directly or indirectly by providing the improper developmental milieu through effects on other cells. We investigated this question using female mice heterozygous for two x chromosomal genes, xid and Pgk-1 (phosphoglycerate kinase-1). Since females are mosaic because of x chromosome inactivation, their lymphocytes can be studied for the choice of the x chromosome, using the two PGK-1 isoenzymes as the cytological marker. We find that B lymphocytes in the spleen prefer the x chromosome without xid while the remaining splenocytes and cells from other tissues do not. This suggests that xid affects B lymphocytes directly and not through their developmental milieu. Furthermore, our data suggest that the precursors for IgG1- and IgG3-producing cells may be both few and different. The Rockefeller University Press 1983-09-01 /pmc/articles/PMC2187116/ /pubmed/6604128 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency
title Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency
title_full Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency
title_fullStr Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency
title_full_unstemmed Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency
title_short Unbalanced X chromosome mosaicism in B cells of mice with X-linked immunodeficiency
title_sort unbalanced x chromosome mosaicism in b cells of mice with x-linked immunodeficiency
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187116/
https://www.ncbi.nlm.nih.gov/pubmed/6604128