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Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells

A low proportion of T lymphocytes in normal mouse spleen contains small intracytoplasmic vesicles showing Class I MHC molecules. After stimulation in vitro in a mixed lymphocyte reaction or by addition of Con A, the proportion of T cells with such intracytoplasmic vesicles increases progressively an...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187213/
https://www.ncbi.nlm.nih.gov/pubmed/6363594
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description A low proportion of T lymphocytes in normal mouse spleen contains small intracytoplasmic vesicles showing Class I MHC molecules. After stimulation in vitro in a mixed lymphocyte reaction or by addition of Con A, the proportion of T cells with such intracytoplasmic vesicles increases progressively and becomes the majority. Labeling with fluorochrome-conjugated antibodies has shown that the vesicles are formed by internalization of molecules from the plasma membrane. The process is spontaneous and does not require cross-linking by antibodies or other ligands; it is selective inasmuch as other molecules (Thy-1 and T200 antigens) are not included and it is specific since it is not performed by other cells such as B lymphoid cells or fibroblasts. On the whole the process shows similarities with the internalization and recycling of other receptors, such as the receptors for different macromolecules of metabolic or informational significance, as seen in other cells. On the other hand, the specificity of Class I MHC mobilization in T lymphoid cells suggest a role for this process which is related to the immune function of these molecules.
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spelling pubmed-21872132008-04-17 Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells J Exp Med Articles A low proportion of T lymphocytes in normal mouse spleen contains small intracytoplasmic vesicles showing Class I MHC molecules. After stimulation in vitro in a mixed lymphocyte reaction or by addition of Con A, the proportion of T cells with such intracytoplasmic vesicles increases progressively and becomes the majority. Labeling with fluorochrome-conjugated antibodies has shown that the vesicles are formed by internalization of molecules from the plasma membrane. The process is spontaneous and does not require cross-linking by antibodies or other ligands; it is selective inasmuch as other molecules (Thy-1 and T200 antigens) are not included and it is specific since it is not performed by other cells such as B lymphoid cells or fibroblasts. On the whole the process shows similarities with the internalization and recycling of other receptors, such as the receptors for different macromolecules of metabolic or informational significance, as seen in other cells. On the other hand, the specificity of Class I MHC mobilization in T lymphoid cells suggest a role for this process which is related to the immune function of these molecules. The Rockefeller University Press 1984-01-01 /pmc/articles/PMC2187213/ /pubmed/6363594 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells
title Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells
title_full Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells
title_fullStr Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells
title_full_unstemmed Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells
title_short Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells
title_sort spontaneous internalization of class i major histocompatibility complex molecules in t lymphoid cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187213/
https://www.ncbi.nlm.nih.gov/pubmed/6363594