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Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies

Although activation of B lymphocytes by antigen or anti-Ig antibody has been shown to require cross-linking of surface Ig molecules, cross- linking is not necessary for B cell activation by anti-Lyb-2 monoclonal antibody (MAb). Monovalent Fab' fragments of anti-Lyb-2 MAb are as effective as the...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187309/
https://www.ncbi.nlm.nih.gov/pubmed/6427385
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description Although activation of B lymphocytes by antigen or anti-Ig antibody has been shown to require cross-linking of surface Ig molecules, cross- linking is not necessary for B cell activation by anti-Lyb-2 monoclonal antibody (MAb). Monovalent Fab' fragments of anti-Lyb-2 MAb are as effective as the intact antibody in inducing blast cell transformation of small B cells and B cell proliferation in the apparent absence of T cells and adherent cells. In the presence of factors from T cells, B cells activated by Fab' fragments of anti-Lyb-2 MAb were induced to mature into Ig-secreting cells. Since monovalent Fab' fragments probably cannot induce receptor aggregation, it appears that receptor occupancy is sufficient to induce B cell activation with anti-Lyb-2 MAb.
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spelling pubmed-21873092008-04-17 Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies J Exp Med Articles Although activation of B lymphocytes by antigen or anti-Ig antibody has been shown to require cross-linking of surface Ig molecules, cross- linking is not necessary for B cell activation by anti-Lyb-2 monoclonal antibody (MAb). Monovalent Fab' fragments of anti-Lyb-2 MAb are as effective as the intact antibody in inducing blast cell transformation of small B cells and B cell proliferation in the apparent absence of T cells and adherent cells. In the presence of factors from T cells, B cells activated by Fab' fragments of anti-Lyb-2 MAb were induced to mature into Ig-secreting cells. Since monovalent Fab' fragments probably cannot induce receptor aggregation, it appears that receptor occupancy is sufficient to induce B cell activation with anti-Lyb-2 MAb. The Rockefeller University Press 1984-06-01 /pmc/articles/PMC2187309/ /pubmed/6427385 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies
title Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies
title_full Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies
title_fullStr Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies
title_full_unstemmed Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies
title_short Activation of B lymphocytes by monovalent anti-Lyb-2 antibodies
title_sort activation of b lymphocytes by monovalent anti-lyb-2 antibodies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187309/
https://www.ncbi.nlm.nih.gov/pubmed/6427385