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Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci
We have investigated the opsonic and protective effects of fibronectin (FN) against type III group B streptococci. When used by itself, the FN failed to promote actual internalization of group B organisms. The addition of FN to group B streptococci that had been preopsonized in an immunoglobulin pre...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1984
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187317/ https://www.ncbi.nlm.nih.gov/pubmed/6374010 |
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collection | PubMed |
description | We have investigated the opsonic and protective effects of fibronectin (FN) against type III group B streptococci. When used by itself, the FN failed to promote actual internalization of group B organisms. The addition of FN to group B streptococci that had been preopsonized in an immunoglobulin preparation modified for intravenous use ( IgIV ) or a type-specific, murine monoclonal antibody of IgG isotype markedly enhanced interaction with human polymorphonuclear leukocytes (PMN). A similar enhanced effect was observed when the FN was combined with type- specific monoclonal antibody preparations of IgM and, surprisingly, IgA isotype. Preincubation experiments indicated that the major effect was upon the PMN rather than directly on the bacteria, but we could not demonstrate an effect of FN on cell surface receptors for the Fc fragment of Ig or C3b using rosetting techniques. In addition to enhancing the in vitro opsonic activity of Ig, the FN significantly increased the protective effect of the polyclonal and monoclonal Ig preparations in an animal model of neonatal group B streptococcal disease. Thus, FN appears to have a critical role in the host defense mechanisms against group B streptococci. |
format | Text |
id | pubmed-2187317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21873172008-04-17 Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci J Exp Med Articles We have investigated the opsonic and protective effects of fibronectin (FN) against type III group B streptococci. When used by itself, the FN failed to promote actual internalization of group B organisms. The addition of FN to group B streptococci that had been preopsonized in an immunoglobulin preparation modified for intravenous use ( IgIV ) or a type-specific, murine monoclonal antibody of IgG isotype markedly enhanced interaction with human polymorphonuclear leukocytes (PMN). A similar enhanced effect was observed when the FN was combined with type- specific monoclonal antibody preparations of IgM and, surprisingly, IgA isotype. Preincubation experiments indicated that the major effect was upon the PMN rather than directly on the bacteria, but we could not demonstrate an effect of FN on cell surface receptors for the Fc fragment of Ig or C3b using rosetting techniques. In addition to enhancing the in vitro opsonic activity of Ig, the FN significantly increased the protective effect of the polyclonal and monoclonal Ig preparations in an animal model of neonatal group B streptococcal disease. Thus, FN appears to have a critical role in the host defense mechanisms against group B streptococci. The Rockefeller University Press 1984-06-01 /pmc/articles/PMC2187317/ /pubmed/6374010 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci |
title | Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci |
title_full | Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci |
title_fullStr | Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci |
title_full_unstemmed | Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci |
title_short | Fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group B streptococci |
title_sort | fibronectin enhances the opsonic and protective activity of monoclonal and polyclonal antibody against group b streptococci |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187317/ https://www.ncbi.nlm.nih.gov/pubmed/6374010 |