Human Ia beta chains and the invariant chain share a common antigenic determinant

We have presented evidence that a mouse monoclonal antibody 1,227, which was previously shown to recognize a determinant on the light chains beta 1 and beta 2 of human Ia antigens, also recognizes a determinant on the invariant chain (1) associated with these molecules. Ia-specific proteins were res...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187380/
https://www.ncbi.nlm.nih.gov/pubmed/6194245
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collection PubMed
description We have presented evidence that a mouse monoclonal antibody 1,227, which was previously shown to recognize a determinant on the light chains beta 1 and beta 2 of human Ia antigens, also recognizes a determinant on the invariant chain (1) associated with these molecules. Ia-specific proteins were resolved by two-dimensional (2D) PAGE and electrophoretically transferred onto nitrocellulose filters. The presence of a determinant shared between beta 1, beta 2 and I was established using "Western blotting" technique. In addition, we demonstrated that 1,227 can immunoprecipitate isolated beta 1, beta 2, and the invariant chain proteins following their separate elution from SDS-PAGE.
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spelling pubmed-21873802008-04-17 Human Ia beta chains and the invariant chain share a common antigenic determinant J Exp Med Articles We have presented evidence that a mouse monoclonal antibody 1,227, which was previously shown to recognize a determinant on the light chains beta 1 and beta 2 of human Ia antigens, also recognizes a determinant on the invariant chain (1) associated with these molecules. Ia-specific proteins were resolved by two-dimensional (2D) PAGE and electrophoretically transferred onto nitrocellulose filters. The presence of a determinant shared between beta 1, beta 2 and I was established using "Western blotting" technique. In addition, we demonstrated that 1,227 can immunoprecipitate isolated beta 1, beta 2, and the invariant chain proteins following their separate elution from SDS-PAGE. The Rockefeller University Press 1983-10-01 /pmc/articles/PMC2187380/ /pubmed/6194245 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Human Ia beta chains and the invariant chain share a common antigenic determinant
title Human Ia beta chains and the invariant chain share a common antigenic determinant
title_full Human Ia beta chains and the invariant chain share a common antigenic determinant
title_fullStr Human Ia beta chains and the invariant chain share a common antigenic determinant
title_full_unstemmed Human Ia beta chains and the invariant chain share a common antigenic determinant
title_short Human Ia beta chains and the invariant chain share a common antigenic determinant
title_sort human ia beta chains and the invariant chain share a common antigenic determinant
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187380/
https://www.ncbi.nlm.nih.gov/pubmed/6194245

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