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Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted
The requirements for T cell/B cell interaction for the induction of primary in vitro antibody responses to phosphorylcholine (PC)-keyhole limpet hemocyanin (KLH) were examined. Long-term helper T cell lines derived from KLH-primed (CBA/N X BALB/c) F1 female mice (H-2k/d) were able to support a T15-i...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1984
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187418/ https://www.ncbi.nlm.nih.gov/pubmed/6204002 |
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collection | PubMed |
description | The requirements for T cell/B cell interaction for the induction of primary in vitro antibody responses to phosphorylcholine (PC)-keyhole limpet hemocyanin (KLH) were examined. Long-term helper T cell lines derived from KLH-primed (CBA/N X BALB/c) F1 female mice (H-2k/d) were able to support a T15-idiotype dominant, IgM anti-PC response of BALB/c (H-2d) B cells and macrophages, but could not activate PC-specific responses by BALB.B (H-2b) B cells, even in the presence of irradiated H-2k/d antigen-presenting cells. Polyclonal IgM secretion in the same cultures did not appear to depend upon a major histocompatibility complex (MHC)-restricted T-B interaction. Since IgM anti-PC responses seem to be entirely derived from the Lyb5+ B cell subpopulation, we conclude that at least some Lyb5+ B cells can only be activated by MHC- restricted T-B interactions. We also found that xid B cells from (CBA/N X BALB/c) F1 male mice could be polyclonally activated by helper T cell lines by an apparently MHC-unrestricted interaction. Our data thus suggests that residence in the Lyb5- or Lyb5+ B cell subset does not determine the T:B interaction requirements for antibody synthesis. |
format | Text |
id | pubmed-2187418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21874182008-04-17 Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted J Exp Med Articles The requirements for T cell/B cell interaction for the induction of primary in vitro antibody responses to phosphorylcholine (PC)-keyhole limpet hemocyanin (KLH) were examined. Long-term helper T cell lines derived from KLH-primed (CBA/N X BALB/c) F1 female mice (H-2k/d) were able to support a T15-idiotype dominant, IgM anti-PC response of BALB/c (H-2d) B cells and macrophages, but could not activate PC-specific responses by BALB.B (H-2b) B cells, even in the presence of irradiated H-2k/d antigen-presenting cells. Polyclonal IgM secretion in the same cultures did not appear to depend upon a major histocompatibility complex (MHC)-restricted T-B interaction. Since IgM anti-PC responses seem to be entirely derived from the Lyb5+ B cell subpopulation, we conclude that at least some Lyb5+ B cells can only be activated by MHC- restricted T-B interactions. We also found that xid B cells from (CBA/N X BALB/c) F1 male mice could be polyclonally activated by helper T cell lines by an apparently MHC-unrestricted interaction. Our data thus suggests that residence in the Lyb5- or Lyb5+ B cell subset does not determine the T:B interaction requirements for antibody synthesis. The Rockefeller University Press 1984-07-01 /pmc/articles/PMC2187418/ /pubmed/6204002 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted |
title | Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted |
title_full | Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted |
title_fullStr | Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted |
title_full_unstemmed | Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted |
title_short | Interaction of helper T cells and Lyb5+ B cells responding to phosphorylcholine is MHC-restricted |
title_sort | interaction of helper t cells and lyb5+ b cells responding to phosphorylcholine is mhc-restricted |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187418/ https://www.ncbi.nlm.nih.gov/pubmed/6204002 |