Isolation, characterization, and propagation in vitro of human glomerular endothelial cells

Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygo...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187431/
https://www.ncbi.nlm.nih.gov/pubmed/6330271
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collection PubMed
description Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygonal cells. Von Willebrand's factor (VWF) was detected in the cytoplasm of all clones. Their intermediate filaments differed antigenically from that present in human umbilical vein endothelial cells. Like other endothelial cells, they demonstrated high levels of membrane-associated angiotensin-converting enzyme (ACE).
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spelling pubmed-21874312008-04-17 Isolation, characterization, and propagation in vitro of human glomerular endothelial cells J Exp Med Articles Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygonal cells. Von Willebrand's factor (VWF) was detected in the cytoplasm of all clones. Their intermediate filaments differed antigenically from that present in human umbilical vein endothelial cells. Like other endothelial cells, they demonstrated high levels of membrane-associated angiotensin-converting enzyme (ACE). The Rockefeller University Press 1984-07-01 /pmc/articles/PMC2187431/ /pubmed/6330271 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Isolation, characterization, and propagation in vitro of human glomerular endothelial cells
title Isolation, characterization, and propagation in vitro of human glomerular endothelial cells
title_full Isolation, characterization, and propagation in vitro of human glomerular endothelial cells
title_fullStr Isolation, characterization, and propagation in vitro of human glomerular endothelial cells
title_full_unstemmed Isolation, characterization, and propagation in vitro of human glomerular endothelial cells
title_short Isolation, characterization, and propagation in vitro of human glomerular endothelial cells
title_sort isolation, characterization, and propagation in vitro of human glomerular endothelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187431/
https://www.ncbi.nlm.nih.gov/pubmed/6330271

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