Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment

A complete set of a rearranged human gamma 1-heavy chain gene, HIG1, was cloned from human plasma cell leukemia line, ARH-77, and transferred into mouse cells. It was strongly expressed in mouse myeloma cells but not in mouse L cells, indicating that immunoglobulin gene expression is not species-spe...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187535/
https://www.ncbi.nlm.nih.gov/pubmed/6512493
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collection PubMed
description A complete set of a rearranged human gamma 1-heavy chain gene, HIG1, was cloned from human plasma cell leukemia line, ARH-77, and transferred into mouse cells. It was strongly expressed in mouse myeloma cells but not in mouse L cells, indicating that immunoglobulin gene expression is not species-specific but cell-specific. However, a remarkable production of human gamma 1 chain was induced in mouse L cells containing HIG1 gene when the cells were treated with cycloheximide for a short period. The role of a labile repressor molecule in the expression of the immunoglobulin gene is proposed.
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spelling pubmed-21875352008-04-17 Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment J Exp Med Articles A complete set of a rearranged human gamma 1-heavy chain gene, HIG1, was cloned from human plasma cell leukemia line, ARH-77, and transferred into mouse cells. It was strongly expressed in mouse myeloma cells but not in mouse L cells, indicating that immunoglobulin gene expression is not species-specific but cell-specific. However, a remarkable production of human gamma 1 chain was induced in mouse L cells containing HIG1 gene when the cells were treated with cycloheximide for a short period. The role of a labile repressor molecule in the expression of the immunoglobulin gene is proposed. The Rockefeller University Press 1984-12-01 /pmc/articles/PMC2187535/ /pubmed/6512493 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
title Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
title_full Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
title_fullStr Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
title_full_unstemmed Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
title_short Induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
title_sort induction of immunoglobulin gene expression in mouse fibroblasts by cycloheximide treatment
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187535/
https://www.ncbi.nlm.nih.gov/pubmed/6512493

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