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Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing sup...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1984
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187537/ https://www.ncbi.nlm.nih.gov/pubmed/6439816 |
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collection | PubMed |
description | To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1- secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion. |
format | Text |
id | pubmed-2187537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21875372008-04-17 Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) J Exp Med Articles To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1- secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion. The Rockefeller University Press 1984-12-01 /pmc/articles/PMC2187537/ /pubmed/6439816 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) |
title | Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) |
title_full | Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) |
title_fullStr | Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) |
title_full_unstemmed | Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) |
title_short | Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) |
title_sort | clonal analysis of b cells induced to secrete igg by t cell-derived lymphokine(s) |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187537/ https://www.ncbi.nlm.nih.gov/pubmed/6439816 |