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Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)

To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing sup...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187537/
https://www.ncbi.nlm.nih.gov/pubmed/6439816
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collection PubMed
description To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1- secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion.
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spelling pubmed-21875372008-04-17 Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s) J Exp Med Articles To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1- secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion. The Rockefeller University Press 1984-12-01 /pmc/articles/PMC2187537/ /pubmed/6439816 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
title Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
title_full Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
title_fullStr Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
title_full_unstemmed Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
title_short Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s)
title_sort clonal analysis of b cells induced to secrete igg by t cell-derived lymphokine(s)
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187537/
https://www.ncbi.nlm.nih.gov/pubmed/6439816