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Multiple, heart-cross-reactive epitopes of streptococcal M proteins

We present evidence that a highly purified pepsin extract of type 5 streptococcal M protein (pep M5) contains at least three epitopes that are cross-reactive with sarcolemmal membrane proteins of human myocardium. The tissue-cross-reactive determinants of pep M5 are also partially shared with pep M6...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1985
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187556/
https://www.ncbi.nlm.nih.gov/pubmed/2578539
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description We present evidence that a highly purified pepsin extract of type 5 streptococcal M protein (pep M5) contains at least three epitopes that are cross-reactive with sarcolemmal membrane proteins of human myocardium. The tissue-cross-reactive determinants of pep M5 are also partially shared with pep M6 and pep M19. Three rabbits immunized with a single 300 micrograms dose of pep M5 developed significant levels of heart-cross-reactive antibodies, as determined by indirect immunofluorescence tests. All three sera also contained antibodies that cross-reacted with pep M6 and pep M19. The heart tissue--specific antibodies that were eluted from sarcolemmal membranes opsonized types 5, 6, and 19 streptococci, indicating that they were directed against protective M protein epitopes on the surface of virulent organisms. Immunofluorescence inhibition tests, using purified M proteins as soluble inhibitors of heart-cross-reactive antibodies, revealed the number and M protein serotype distribution of the tissue-cross-reactive epitopes. Immunoblot analyses demonstrated the sarcolemmal membrane proteins containing the various cross-reactive antigenic determinants.
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spelling pubmed-21875562008-04-17 Multiple, heart-cross-reactive epitopes of streptococcal M proteins J Exp Med Articles We present evidence that a highly purified pepsin extract of type 5 streptococcal M protein (pep M5) contains at least three epitopes that are cross-reactive with sarcolemmal membrane proteins of human myocardium. The tissue-cross-reactive determinants of pep M5 are also partially shared with pep M6 and pep M19. Three rabbits immunized with a single 300 micrograms dose of pep M5 developed significant levels of heart-cross-reactive antibodies, as determined by indirect immunofluorescence tests. All three sera also contained antibodies that cross-reacted with pep M6 and pep M19. The heart tissue--specific antibodies that were eluted from sarcolemmal membranes opsonized types 5, 6, and 19 streptococci, indicating that they were directed against protective M protein epitopes on the surface of virulent organisms. Immunofluorescence inhibition tests, using purified M proteins as soluble inhibitors of heart-cross-reactive antibodies, revealed the number and M protein serotype distribution of the tissue-cross-reactive epitopes. Immunoblot analyses demonstrated the sarcolemmal membrane proteins containing the various cross-reactive antigenic determinants. The Rockefeller University Press 1985-01-01 /pmc/articles/PMC2187556/ /pubmed/2578539 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Multiple, heart-cross-reactive epitopes of streptococcal M proteins
title Multiple, heart-cross-reactive epitopes of streptococcal M proteins
title_full Multiple, heart-cross-reactive epitopes of streptococcal M proteins
title_fullStr Multiple, heart-cross-reactive epitopes of streptococcal M proteins
title_full_unstemmed Multiple, heart-cross-reactive epitopes of streptococcal M proteins
title_short Multiple, heart-cross-reactive epitopes of streptococcal M proteins
title_sort multiple, heart-cross-reactive epitopes of streptococcal m proteins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187556/
https://www.ncbi.nlm.nih.gov/pubmed/2578539