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Cellular basis of the proliferative response of human T cells to mouse xenoantigens

Purified human T cells respond proliferatively to allogenic peripheral blood mononuclear (PBMC) stimulating cells but show no response to murine splenic stimulating cells. Two possible explanations for the lack of xenogeneic response are that human T cells, educated in a human thymus, cannot directl...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187659/
https://www.ncbi.nlm.nih.gov/pubmed/2295880
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description Purified human T cells respond proliferatively to allogenic peripheral blood mononuclear (PBMC) stimulating cells but show no response to murine splenic stimulating cells. Two possible explanations for the lack of xenogeneic response are that human T cells, educated in a human thymus, cannot directly recognize a molecule as disparate as mouse antigen encoded by H-2 and/or that a cytokine(s) produced by the APCs is needed to allow a proliferative response and that the cytokine(s) produced by murine APC do not provide an adequate stimulus to the human T cells under these conditions. We show here that highly purified human T cells can respond directly in an antigen-specific manner to murine stimulating cells if human rIL-1 or rIL-2 or a T cell growth factor (TCGF) preparation are present in the culture. These findings demonstrate that human T cells can recognize murine antigens and that a highly significant response can be obtained if a human cytokine is present to permit that response.
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spelling pubmed-21876592008-04-17 Cellular basis of the proliferative response of human T cells to mouse xenoantigens J Exp Med Articles Purified human T cells respond proliferatively to allogenic peripheral blood mononuclear (PBMC) stimulating cells but show no response to murine splenic stimulating cells. Two possible explanations for the lack of xenogeneic response are that human T cells, educated in a human thymus, cannot directly recognize a molecule as disparate as mouse antigen encoded by H-2 and/or that a cytokine(s) produced by the APCs is needed to allow a proliferative response and that the cytokine(s) produced by murine APC do not provide an adequate stimulus to the human T cells under these conditions. We show here that highly purified human T cells can respond directly in an antigen-specific manner to murine stimulating cells if human rIL-1 or rIL-2 or a T cell growth factor (TCGF) preparation are present in the culture. These findings demonstrate that human T cells can recognize murine antigens and that a highly significant response can be obtained if a human cytokine is present to permit that response. The Rockefeller University Press 1990-01-01 /pmc/articles/PMC2187659/ /pubmed/2295880 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Cellular basis of the proliferative response of human T cells to mouse xenoantigens
title Cellular basis of the proliferative response of human T cells to mouse xenoantigens
title_full Cellular basis of the proliferative response of human T cells to mouse xenoantigens
title_fullStr Cellular basis of the proliferative response of human T cells to mouse xenoantigens
title_full_unstemmed Cellular basis of the proliferative response of human T cells to mouse xenoantigens
title_short Cellular basis of the proliferative response of human T cells to mouse xenoantigens
title_sort cellular basis of the proliferative response of human t cells to mouse xenoantigens
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187659/
https://www.ncbi.nlm.nih.gov/pubmed/2295880