High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors

125I-labeled TNF(LuKII) (tumor necrosis factor) binds specifically to human and mouse cell lines sensitive to the cytotoxic effect of TNF, but not to cells made resistant to TNF. TNF-sensitive cells have cell surface receptors with a high affinity for TNF(LuKII). Mouse TNF competes with TNF(LuKII) f...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1985
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187809/
https://www.ncbi.nlm.nih.gov/pubmed/2993471
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collection PubMed
description 125I-labeled TNF(LuKII) (tumor necrosis factor) binds specifically to human and mouse cell lines sensitive to the cytotoxic effect of TNF, but not to cells made resistant to TNF. TNF-sensitive cells have cell surface receptors with a high affinity for TNF(LuKII). Mouse TNF competes with TNF(LuKII) for receptor binding. Scatchard analysis of the binding data yielded linear plots and suggests that TNF(LuKII) binds to homogeneous receptor sites. The number of TNF(LuKII) receptors on two TNF-sensitive cell lines is 200-300 per cell and the affinity constant of the receptor for TNF(LuKII) is approximately 1 X 10(-10) M.
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spelling pubmed-21878092008-04-17 High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors J Exp Med Articles 125I-labeled TNF(LuKII) (tumor necrosis factor) binds specifically to human and mouse cell lines sensitive to the cytotoxic effect of TNF, but not to cells made resistant to TNF. TNF-sensitive cells have cell surface receptors with a high affinity for TNF(LuKII). Mouse TNF competes with TNF(LuKII) for receptor binding. Scatchard analysis of the binding data yielded linear plots and suggests that TNF(LuKII) binds to homogeneous receptor sites. The number of TNF(LuKII) receptors on two TNF-sensitive cell lines is 200-300 per cell and the affinity constant of the receptor for TNF(LuKII) is approximately 1 X 10(-10) M. The Rockefeller University Press 1985-09-01 /pmc/articles/PMC2187809/ /pubmed/2993471 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors
title High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors
title_full High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors
title_fullStr High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors
title_full_unstemmed High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors
title_short High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors
title_sort high affinity binding of 125i-labeled human tumor necrosis factor (lukii) to specific cell surface receptors
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187809/
https://www.ncbi.nlm.nih.gov/pubmed/2993471

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