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Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells

The TCR/CD3 complex plays a central role in antigen recognition and activation of mature T cells, and, therefore, abnormalities in the expression of the complex should induce unresponsiveness of T cells to antigen stimulus. Using flow cytometry, we detected and enumerated variant cells with loss or...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187968/
https://www.ncbi.nlm.nih.gov/pubmed/1972177
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description The TCR/CD3 complex plays a central role in antigen recognition and activation of mature T cells, and, therefore, abnormalities in the expression of the complex should induce unresponsiveness of T cells to antigen stimulus. Using flow cytometry, we detected and enumerated variant cells with loss or alteration of the surface TCR/CD3 expression among human mature CD4+ T cells. The presence of variant CD4+ T cells was demonstrated by isolating and cloning them from peripheral blood, and their abnormalities can be accounted for by alterations in TCR expression such as defects of protein expression and partial protein deletion. The variant frequency in peripheral blood increased with aging in normal donors and was highly elevated in patients with ataxia telangiectasia, an autosomal recessive inherited disease with defective DNA repair and variable T cell immunodeficiency. These findings suggest that such alterations in TCR expression are induced by somatic mutagenesis of TCR genes and can be important factors related to age- dependent and genetic disease-associated T cell dysfunction.
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spelling pubmed-21879682008-04-17 Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells J Exp Med Articles The TCR/CD3 complex plays a central role in antigen recognition and activation of mature T cells, and, therefore, abnormalities in the expression of the complex should induce unresponsiveness of T cells to antigen stimulus. Using flow cytometry, we detected and enumerated variant cells with loss or alteration of the surface TCR/CD3 expression among human mature CD4+ T cells. The presence of variant CD4+ T cells was demonstrated by isolating and cloning them from peripheral blood, and their abnormalities can be accounted for by alterations in TCR expression such as defects of protein expression and partial protein deletion. The variant frequency in peripheral blood increased with aging in normal donors and was highly elevated in patients with ataxia telangiectasia, an autosomal recessive inherited disease with defective DNA repair and variable T cell immunodeficiency. These findings suggest that such alterations in TCR expression are induced by somatic mutagenesis of TCR genes and can be important factors related to age- dependent and genetic disease-associated T cell dysfunction. The Rockefeller University Press 1990-06-01 /pmc/articles/PMC2187968/ /pubmed/1972177 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells
title Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells
title_full Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells
title_fullStr Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells
title_full_unstemmed Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells
title_short Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells
title_sort spontaneous loss and alteration of antigen receptor expression in mature cd4+ t cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2187968/
https://www.ncbi.nlm.nih.gov/pubmed/1972177