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Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens

IFN-gamma is known to induce the expression of Ia antigens on macrophages. We found that murine IFN-alpha and -beta blocked the effects of IFN-gamma in a dose-dependent manner. The antagonistic effect of IFN-alpha and -beta was observed even when macrophages were prestimulated with IFN-gamma. These...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188081/
https://www.ncbi.nlm.nih.gov/pubmed/2419472
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description IFN-gamma is known to induce the expression of Ia antigens on macrophages. We found that murine IFN-alpha and -beta blocked the effects of IFN-gamma in a dose-dependent manner. The antagonistic effect of IFN-alpha and -beta was observed even when macrophages were prestimulated with IFN-gamma. These inhibitory effects of IFN-alpha or - beta were blocked by their respective antibodies. The block exerted by IFN-alpha/beta was similar whether Ia levels were monitored by immunofluorescence with anti-Ia mAb, or by stimulation of freshly sensitized, alloreactive T lymphoblasts. Adherent macrophage-rich populations from newborn mice were incapable of expressing Ia antigens following stimulation with IFN-gamma, and would inhibit the response of adult macrophages to this lymphokine. Addition of anti-IFN-beta mAb, but not anti-IFN-alpha allowed newborns' macrophages to express Ia in response to IFN-gamma, and ablated the suppressive activity toward adult cells. These results indicate that IFN-alpha and -beta, which can be produced in the course of self-defense responses and during ontogeny, may contribute to the down-regulation of macrophage Ia expression.
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spelling pubmed-21880812008-04-17 Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens J Exp Med Articles IFN-gamma is known to induce the expression of Ia antigens on macrophages. We found that murine IFN-alpha and -beta blocked the effects of IFN-gamma in a dose-dependent manner. The antagonistic effect of IFN-alpha and -beta was observed even when macrophages were prestimulated with IFN-gamma. These inhibitory effects of IFN-alpha or - beta were blocked by their respective antibodies. The block exerted by IFN-alpha/beta was similar whether Ia levels were monitored by immunofluorescence with anti-Ia mAb, or by stimulation of freshly sensitized, alloreactive T lymphoblasts. Adherent macrophage-rich populations from newborn mice were incapable of expressing Ia antigens following stimulation with IFN-gamma, and would inhibit the response of adult macrophages to this lymphokine. Addition of anti-IFN-beta mAb, but not anti-IFN-alpha allowed newborns' macrophages to express Ia in response to IFN-gamma, and ablated the suppressive activity toward adult cells. These results indicate that IFN-alpha and -beta, which can be produced in the course of self-defense responses and during ontogeny, may contribute to the down-regulation of macrophage Ia expression. The Rockefeller University Press 1986-04-01 /pmc/articles/PMC2188081/ /pubmed/2419472 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens
title Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens
title_full Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens
title_fullStr Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens
title_full_unstemmed Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens
title_short Contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage Ia antigens
title_sort contrasting effect of alpha/beta- and gamma-interferons on expression of macrophage ia antigens
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188081/
https://www.ncbi.nlm.nih.gov/pubmed/2419472