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Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells
A purified murine lymphokine, eosinophil differentiation factor (EDF), was found to be a selective stimulus for the clonal proliferation and differentiation of murine eosinophil progenitor cells, establishing it as the murine eosinophil colony-stimulating factor (Eo-CSF). EDF was also active on huma...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1986
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188112/ https://www.ncbi.nlm.nih.gov/pubmed/3486243 |
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collection | PubMed |
description | A purified murine lymphokine, eosinophil differentiation factor (EDF), was found to be a selective stimulus for the clonal proliferation and differentiation of murine eosinophil progenitor cells, establishing it as the murine eosinophil colony-stimulating factor (Eo-CSF). EDF was also active on human eosinophil progenitors and mature blood eosinophils, but had no effect on neutrophil or macrophage precursor cells, nor on blood neutrophils. In culture of human bone marrow cells, EDF stimulated equal numbers and equal sizes of eosinophil colonies to develop when compared with human placental conditioned medium, a source of human CSFs, suggesting that all responsive progenitor cells were stimulated. Clone transfer experiments and the linear relationship between number of bone marrow cells plated and colonies produced confirmed that the action of EDF was directly on eosinophil progenitor cells. EDF increased the capacity of human blood eosinophils, but not neutrophils, to kill antibody-coated tumor cells and to phagocytose serum-opsonized yeast cells. This functional activation was associated with the enhanced expression of functional antigens (GFA-1, GFA-2, and the receptor for C3bi) on eosinophils. The possession by EDF (Eo-CSF) of all the properties expected of a human eosinophil CSF raises the possibility that a human analog of this molecule exists, and is involved in the regulation of production and function of human eosinophils in vivo. |
format | Text |
id | pubmed-2188112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1986 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21881122008-04-17 Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells J Exp Med Articles A purified murine lymphokine, eosinophil differentiation factor (EDF), was found to be a selective stimulus for the clonal proliferation and differentiation of murine eosinophil progenitor cells, establishing it as the murine eosinophil colony-stimulating factor (Eo-CSF). EDF was also active on human eosinophil progenitors and mature blood eosinophils, but had no effect on neutrophil or macrophage precursor cells, nor on blood neutrophils. In culture of human bone marrow cells, EDF stimulated equal numbers and equal sizes of eosinophil colonies to develop when compared with human placental conditioned medium, a source of human CSFs, suggesting that all responsive progenitor cells were stimulated. Clone transfer experiments and the linear relationship between number of bone marrow cells plated and colonies produced confirmed that the action of EDF was directly on eosinophil progenitor cells. EDF increased the capacity of human blood eosinophils, but not neutrophils, to kill antibody-coated tumor cells and to phagocytose serum-opsonized yeast cells. This functional activation was associated with the enhanced expression of functional antigens (GFA-1, GFA-2, and the receptor for C3bi) on eosinophils. The possession by EDF (Eo-CSF) of all the properties expected of a human eosinophil CSF raises the possibility that a human analog of this molecule exists, and is involved in the regulation of production and function of human eosinophils in vivo. The Rockefeller University Press 1986-05-01 /pmc/articles/PMC2188112/ /pubmed/3486243 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells |
title | Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells |
title_full | Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells |
title_fullStr | Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells |
title_full_unstemmed | Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells |
title_short | Murine eosinophil differentiation factor. An eosinophil-specific colony- stimulating factor with activity for human cells |
title_sort | murine eosinophil differentiation factor. an eosinophil-specific colony- stimulating factor with activity for human cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188112/ https://www.ncbi.nlm.nih.gov/pubmed/3486243 |